Anti-Glutamic Acid Decarboxylase 67 (GAD67) ELISA Kit for Mouse

Intended Uses: This Anti-DsDNA ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Anti-DsDNA in the sample, this Anti-DsDNA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Anti-DsDNA concentration. The concentration of Anti-DsDNA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This Anti-DsDNA enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for Anti-DsDNA. Standards or samples are then added to the microtiter plate wells and Anti-DsDNA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Anti-DsDNA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Anti-DsDNA are added to each well to "sandwich" the Anti-DsDNA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain Anti-DsDNA and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Small volumes of GAD67 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS721437 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Glutamic Acid Decarboxylase 67 antibody (GAD67) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GAD67. The ELISA analytical biochemical technique of the MBS721437 kit is based on GAD67 antibody-GAD67 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GAD67 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GAD67. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).