Corticotropin Releasing Hormone Receptor (CRH-R) ELISA Kit for Mouse

Name: Mouse CRH-R ELISA Kit; Corticotropin Releasing Hormone Receptor; MyBioSource
Brand: MyBioSource
SKU: MBS721586
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS721586

Intended Uses: This CRHR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CRHR. This ELISA kit for research use only, not for therapeutic or test applications!Principle of the Assay: CRHR ELISA kit applies the… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Corticotropin Releasing Hormone Receptor (CRH-R), ELISA Kit

Full Product Name

Mouse Corticotropin Releasing Hormone Receptor ELISA Kit

Product Gene Name

CRH-R elisa kit

Species Reactivity

Mouse

Assay Type

Quantitative Competitive

Samples

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Sensitivity

0.1 ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of CRHR. No significant cross-reactivity or interference between CRHR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRHR and all the analogues, therefore, cross reaction may still exist in some cases.

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

28,122 Da

OMIM

122561

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Product Categories/Family for CRH-R elisa kit

Signal Transduction

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of CRH-R elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CRH-R purchase

MBS721586 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Corticotropin Releasing Hormone Receptor (CRH-R) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CRH-R. The ELISA analytical biochemical technique of the MBS721586 kit is based on CRH-R antibody-CRH-R antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRH-R antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRH-R. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CRH-R. It may not necessarily be applicable to this product.

NCBI GI #

372466597

NCBI GeneID

1394

NCBI Accession #

NP_001243228.1

UniProt Secondary Accession #

Q13008; Q4QRJ1; Q9UK64; B4DIE9

UniProt Related Accession #

P34998

GenBank Nucleotide #

NM_001256299.1

NCBI Official Full Name

corticotropin-releasing factor receptor 1 isoform 5

NCBI Official Synonym Full Names

corticotropin releasing hormone receptor 1

NCBI Official Symbol

CRHR1

NCBI Official Synonym Symbols

CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRHR1f; CRF-R-1; CRH-R-1; CRH-R1h

UniProt Protein Name

Corticotropin-releasing factor receptor 1

UniProt Synonym Protein Names

Corticotropin-releasing hormone receptor 1; CRH-R-1; CRH-R1

Protein Family

Corticotropin-releasing factor receptor

UniProt Gene Name

CRHR1

UniProt Synonym Gene Names

CRFR; CRFR1; CRHR; CRF-R-1; CRF-R1; CRFR-1; CRH-R-1; CRH-R1

UniProt Entry Name

CRFR1_HUMAN

NCBI Protein Information

corticotropin-releasing factor receptor 1; CRH receptor 1 variant B; seven transmembrane helix receptor; corticotropin-releasing hormone receptor 1; corticotropin-releasing factor type 1 receptor; corticotropin releasing hormone receptor variant 1e; corticotropin releasing hormone receptor variant 1g

NCBI Summary for CRH-R

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants, one of which represents a read-through transcript with the neighboring gene MGC57346. [provided by RefSeq, Jan 2012]

UniProt Comments for CRH-R

CRF-R1: a G-protein coupled receptor that specifically binds corticotropin-releasing hormone, a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress. Four splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 2

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: multivesicular body; cell soma; integral to plasma membrane; apical part of cell; dendrite; integral to membrane; plasma membrane; trans-Golgi network; intrinsic to plasma membrane; vesicle

Molecular Function: protein binding; corticotropin-releasing hormone binding; corticotropin-releasing hormone receptor activity; protein complex binding; G-protein alpha-subunit binding; corticotrophin-releasing factor receptor activity

Biological Process: fear response; hypothalamus development; hormone-mediated signaling; adrenal gland development; adenylate cyclase activation; positive regulation of mast cell degranulation; behavioral response to ethanol; female pregnancy; memory; general adaptation syndrome, behavioral process; behavioral response to pain; elevation of cytosolic calcium ion concentration; epithelial cell differentiation; neuropeptide signaling pathway; adrenocorticotropin hormone secretion; behavioral response to cocaine; response to hypoxia; negative regulation of epinephrine secretion; visual learning; immune response; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); parturition; response to electrical stimulus

Research Articles on CRH-R

1. The receptors' binding specificity and structural studies were performed using full-length human CRFR1alpha and mouse CRFR2beta as well as fragments lacking the N-terminal extracellular domain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CRH-R elisa kit

Reference Product	PubMed Publications
CRH elisa kit	>169 publications with CRH-R and CRH
POMC elisa kit	>30 publications with CRH-R and POMC
CRHBP elisa kit	>18 publications with CRH-R and CRHBP
UCN elisa kit	>17 publications with CRH-R and UCN
UCN2 elisa kit	>7 publications with CRH-R and UCN2
UCN3 elisa kit	>6 publications with CRH-R and UCN3
CREB1 elisa kit	>2 publications with CRH-R and CREB1
CALM1 elisa kit	>1 publications with CRH-R and CALM1
SLC2A1 elisa kit	>1 publications with CRH-R and SLC2A1
TAC1 elisa kit	>1 publications with CRH-R and TAC1

Pathways associated with CRH-R elisa kit

Products by Pathway	Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
Corticotropin-releasing Hormone Pathway antibodies	Corticotropin-releasing Hormone Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class B Secretin-like Pathway antibodies	GPCRs, Class B Secretin-like Pathway Diagram
Long-term Depression Pathway antibodies	Long-term Depression Pathway Diagram
Long-term Depression Pathway antibodies	Long-term Depression Pathway Diagram
Myometrial Relaxation And Contraction Pathways antibodies	Myometrial Relaxation And Contraction Pathways Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with CRH-R elisa kit

Disease Name	Pubmed Publications
Anxiety Disorders Antibodies	>39 publications with CRH-R and Anxiety Disorders
Depressive Disorder Antibodies	>35 publications with CRH-R and Depressive Disorder
Depressive Disorder, Major Antibodies	>25 publications with CRH-R and Depressive Disorder, Major
Nervous System Diseases Antibodies	>23 publications with CRH-R and Nervous System Diseases
Disease Models, Animal Antibodies	>17 publications with CRH-R and Disease Models, Animal
Lung Diseases Antibodies	>16 publications with CRH-R and Lung Diseases
Brain Diseases Antibodies	>15 publications with CRH-R and Brain Diseases
Cardiovascular Diseases Antibodies	>10 publications with CRH-R and Cardiovascular Diseases
Anoxia Antibodies	>7 publications with CRH-R and Anoxia
Movement Disorders Antibodies	>7 publications with CRH-R and Movement Disorders

Organs/Tissues associated with CRH-R elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>147 publications with CRH-R and Brain
Embryonic Tissue Antibodies	>5 publications with CRH-R and Embryonic Tissue
Ovary Antibodies	>3 publications with CRH-R and Ovary
Eye Antibodies	>2 publications with CRH-R and Eye