Mullerian Hormone (AMH) ELISA Kit for Mouse

Principle of the Assay: AMH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AMH antibody and an AMH-HRP conjugate. The assay sample and buffer are incubated together with AMH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMH concentration since AMH from samples and AMH-HRP conjugate compete for the anti-AMH antibody binding site. Since the number of sites is limited, as more sites are occupied by AMH from the sample, fewer sites are left to bind AMH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMH concentration in each sample is interpolated from this standard curve.

Intended Uses: This AMH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AMH. This ELISA kit for research use only, not for therapeutic or test applications!

Small volumes of AMH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS722310 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mullerian Hormone (AMH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AMH. The ELISA analytical biochemical technique of the MBS722310 kit is based on AMH antibody-AMH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AMH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AMH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). When you need to drive sex selection in your mouse colony, you need to know the level of anti mullerian hormone AMH. MyBiosource answers your assay issues with our AMH Gen II ELISA kit. With high specificity and excellent inter assay and intra assay reproducibility, our ELISA kits make ICC IHC IHC WB ELISA IP comparisons in your ovarian samples easy. Our AMH ELISA kit is shipped complete for rapid serum sample concentration testing with antibody bound plate, secondary antibodies conjugated to detection reagent, assay diluent, wash and stop solution and standard curve protein for AMH values determination. Each AMH gen II kit is carefully screened with 3 samples middle range to characterize assay precision precision rates, so you know your results will match PubMed cross ref papers. Order as many times as possible. Our hormone AMH ELISA and antibody ant-mullerian hormone are always in stock. MyBiosource.com.