Heat Shock Protein Glycoprotein 96 (HSP gp96) ELISA Kit for Mouse

Principle of the assay: HSP-gp96 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSP-gp96 antibody and an HSP-gp96-HRP conjugate. The assay sample and buffer are incubated together with HSP-gp96-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP-gp96 concentration since HSP-gp96 from samples and HSP-gp96-HRP conjugate compete for the anti-HSP-gp96 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP-gp96 from the sample, fewer sites are left to bind HSP-gp96-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP-gp96 concentration in each sample is interpolated from this standard curve.

Small volumes of HSP gp96 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS722846 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heat Shock Protein glycoprotein 96 (HSP gp96) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSP gp96. The ELISA analytical biochemical technique of the MBS722846 kit is based on HSP gp96 antibody-HSP gp96 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSP gp96 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSP gp96. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).