Procollagen Type III N Terminal Pro (P III NP) ELISA Kit for Human

Principle of the Assay: PIIINP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PIIINP. Standards or samples are then added to the microtiter plate wells and PIIINP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PIIINP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PIIINP are added to each well to "sandwich" the PIIINP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PIIINP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIIINP concentration in each sample is interpolated from this standard curve.

Intended Uses: This PIIINP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PIIINP. This ELISA kit for research use only, not for therapeutic or test applications!

Small volumes of P III NP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS723218 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Procollagen Type III N Terminal Propeptide (P III NP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing P III NP. The ELISA analytical biochemical technique of the MBS723218 kit is based on P III NP antibody-P III NP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect P III NP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, P III NP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Clearly under appreciated, procollagen type and procollagen III are the precursors to the glue that holds us together. Cleaved to form collagen proteins, procollagen III terminal propeptide detection in human samples is quickly done with our precision procollagen type III ELISA kit. Our antibody procollagen III pairs have been tested by SDS Page WB for size and reactivity. Use our ELISA kits to compare your cell IHC assay results to quantifiable read outs - application details are found at Mybiosource.com. Besides, our kits procollagen, view our related propeptide PIIINP assays, including antibodies for PIIINP WB ELISA IP and IHC. Like procollagen III propeptide kits, propeptide PIIINP ELISA has the antibodies size and reactivity validated by PIIINP WB ICC IHC applications. Read Pubmed selected articles using our products at Mybiosource.com.