Myeloperoxidase Antineutrophil Cytoplasmic Antibody (IgG) (MPO ANCA IgG) ELISA Kit for Human

Intended Uses: This MPO-ANCAb IgG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MPO-ANCAb IgG. This ELISA kit for research use only!

Principle of the Assay: MPO-ANCAb IgG ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-MPO-ANCAb IgG antibody and an MPO-ANCAb IgG-HRP conjugate. The assay sample and buffer are incubated together with MPO-ANCAb IgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MPO-ANCAb IgG concentration since MPO-ANCAb IgG from samples and MPO-ANCAb IgG-HRP conjugate compete for the anti-MPO-ANCAb IgG antibody binding site. Since the number of sites is limited, as more sites are occupied by MPO-ANCAb IgG from the sample, fewer sites are left to bind MPO-ANCAb IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MPO-ANCAb IgG concentration in each sample is interpolated from this standard curve.

Small volumes of MPO ANCA IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS723395 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myeloperoxidase antineutrophil cytoplasmic antibody (IgG) (MPO ANCA IgG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MPO ANCA IgG. The ELISA analytical biochemical technique of the MBS723395 kit is based on MPO ANCA IgG antibody-MPO ANCA IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MPO ANCA IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MPO ANCA IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).