Platelet Activating Factor Receptor (PAFR) ELISA Kit for Human

Name: Human PAFR ELISA Kit; Platelet Activating Factor Receptor; MyBioSource
Brand: MyBioSource
SKU: MBS724013
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS724013

Principle of the Assay: PAFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAFR antibody and an PAFR-HRP conjugate. The assay sample and buffer are incubated together with PAFR-HRP conjugate in pre-coated plate for… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Platelet activating factor receptor (PAFR), ELISA Kit

Full Product Name

Human Platelet activating factor receptor ELISA Kit

Product Gene Name

PAFR elisa kit

Species Reactivity

Human

Assay Type

Competitive

Samples

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Detection Range

2.5-50ng/mL

Sensitivity

0.1ng/mL

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

39,203 Da

OMIM

173393

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Intended Uses

This PAFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PAFR. This ELISA kit for research use only, not for therapeutic applications!

Product Categories/Family for PAFR elisa kit

Cardiovascular

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of PAFR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAFR purchase

MBS724013 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Platelet activating factor receptor (PAFR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PAFR. The ELISA analytical biochemical technique of the MBS724013 kit is based on PAFR antibody-PAFR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAFR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAFR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PAFR. It may not necessarily be applicable to this product.

NCBI GI #

257900493

NCBI GeneID

5724

NCBI Accession #

NP_001158195.1

UniProt Secondary Accession #

A3KMC8; A8K2H5

UniProt Related Accession #

P25105

GenBank Nucleotide #

NM_001164723.2

NCBI Official Full Name

platelet-activating factor receptor

NCBI Official Synonym Full Names

platelet-activating factor receptor

NCBI Official Symbol

PTAFR

NCBI Official Synonym Symbols

PAFR

UniProt Protein Name

Platelet-activating factor receptor

Protein Family

Platelet-activating factor receptor

UniProt Gene Name

PTAFR

UniProt Synonym Gene Names

PAFR; PAF-R; PAFr

UniProt Entry Name

PTAFR_HUMAN

NCBI Protein Information

platelet-activating factor receptor; PAF-R

NCBI Summary for PAFR

This gene encodes a seven-transmembrane G-protein-coupled receptor for platelet-activating factor (PAF) that localizes to lipid rafts and/or caveolae in the cell membrane. PAF (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is a phospholipid that plays a significant role in oncogenic transformation, tumor growth, angiogenesis, metastasis, and pro-inflammatory processes. Binding of PAF to the PAF-receptor (PAFR) stimulates numerous signal transduction pathways including phospholipase C, D, A2, mitogen-activated protein kinases (MAPKs), and the phosphatidylinositol-calcium second messenger system. Following PAFR activation, cells become rapidly desensitized and this refractory state is dependent on PAFR phosphorylation, internalization, and down-regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

UniProt Comments for PAFR

PTAFR: Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth- muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Receptor, GPCR

Chromosomal Location of Human Ortholog: 1p35-p34.3

Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; platelet activating factor receptor activity; purinergic nucleotide receptor activity, G-protein coupled; lipopolysaccharide binding; phospholipid binding; lipopolysaccharide receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; phosphoinositide-mediated signaling; inositol trisphosphate biosynthetic process; cytokine and chemokine mediated signaling pathway; lipopolysaccharide-mediated signaling pathway; cytokine production; immune response; inflammatory response; chemotaxis

Research Articles on PAFR

1. patients presenting elevated PAFR expression had significantly longer survival times compared to those with low PAFR expression (log-rank test, p<0.001).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PAFR elisa kit

Products by Pathway	Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram

Diseases associated with PAFR elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>4 publications with PAFR and Inflammation
Cardiovascular Diseases Antibodies	>3 publications with PAFR and Cardiovascular Diseases
Heart Diseases Antibodies	>2 publications with PAFR and Heart Diseases
Fibrosis Antibodies	>2 publications with PAFR and Fibrosis
Liver Diseases Antibodies	>2 publications with PAFR and Liver Diseases
Disease Models, Animal Antibodies	>2 publications with PAFR and Disease Models, Animal
Arteriosclerosis Antibodies	>1 publications with PAFR and Arteriosclerosis
Myocardial Infarction Antibodies	>1 publications with PAFR and Myocardial Infarction
Atherosclerosis Antibodies	>1 publications with PAFR and Atherosclerosis
Necrosis Antibodies	>1 publications with PAFR and Necrosis

Organs/Tissues associated with PAFR elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>6 publications with PAFR and Blood
Uterus Antibodies	>3 publications with PAFR and Uterus
Ovary Antibodies	>1 publications with PAFR and Ovary
Heart Antibodies	>1 publications with PAFR and Heart