Podoplanin (PDPN) ELISA Kit for Human

Name: Human PDPN ELISA Kit; Podoplanin; MyBioSource
Brand: MyBioSource
SKU: MBS724724
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS724724

Principle of the Assay: PDPN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PDPN. Standards or samples are then added to the microtiter plate wells and… More details

$580 / 48-Strip-Wells-(Competitive) +1 FREE 8GB USB

Product Documentation

Sample Competitive Manual Insert View PDF

Sample Sandwich Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Podoplanin (PDPN), ELISA Kit

Full Product Name

Human Podoplanin ELISA Kit

Product Gene Name

PDPN elisa kit

Species Reactivity

Human

Assay Type

Competitive or Sandwich

Samples

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Detection Range

0.5-10ng/mL

Sensitivity

0.1ng/mL

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

12,421 Da

OMIM

608863

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Intended Uses

This PDPN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PDPN. This ELISA kit for research use only, not for therapeutic applications!

Product Categories/Family for PDPN elisa kit

Cardiovascular

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of PDPN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDPN purchase

MBS724724 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Podoplanin (PDPN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDPN. The ELISA analytical biochemical technique of the MBS724724 kit is based on PDPN antibody-PDPN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDPN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDPN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PDPN. It may not necessarily be applicable to this product.

NCBI GI #

21666312

NCBI Accession #

AAM73655.1

UniProt Secondary Accession #

O60836; O95128; Q7L375; Q8NBQ8; Q8NBR3; A9Z1Y2; B2R6J8; E9PB68; F6QWX5

NCBI Official Full Name

podoplanin

UniProt Protein Name

Podoplanin

UniProt Synonym Protein Names

Aggrus; Glycoprotein 36; Gp36; PA2.26 antigen; T1-alpha; T1A

Protein Family

Podoplanin

UniProt Gene Name

PDPN

UniProt Synonym Gene Names

GP36; PSEC0003; PSEC0025; Gp36; T1A

UniProt Entry Name

PDPN_HUMAN

NCBI Summary for PDPN

This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

UniProt Comments for PDPN

PDPN: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. Belongs to the podoplanin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: ruffle; filopodium membrane; lamellipodium; integral to plasma membrane; microvillus membrane; apical plasma membrane; plasma membrane; filopodium; external side of plasma membrane

Molecular Function: folic acid transporter activity; water transporter activity; water channel activity; amino acid transmembrane transporter activity

Biological Process: nervous system development; water transport; cell morphogenesis; positive regulation of cell motility; signal transduction; folic acid transport; prostaglandin metabolic process; regulation of cell shape; cell proliferation; cell-cell adhesion; response to hyperoxia; amino acid transport; lymphangiogenesis; tube morphogenesis; inflammatory response; alveolus development; positive regulation of cell migration; lung development

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.