Angiotensin II Converting Enzyme (ACE II) ELISA Kit for Mouse

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq]

Function: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Ref.1 Ref.2 Ref.14

Catalytic activity: Angiotensin II + H2O = angiotensin-1-7 + L-phenylalanine.

Cofactor: Binds 1 zinc ion per subunit. Ref.13Binds 1 chloride ion per subunit. Ref.13

Enzyme regulation: Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Ref.1 Ref.2 Ref.3 Ref.13

Subunit structure: Interacts with ITGB1. Interacts with SARS-CoV and HCoV-NL63 spike glycoprotein. Ref.11 Ref.14 Ref.17 Ref.20 Ref.22

Subcellular location: Processed angiotensin-converting enzyme 2: Secreted Ref.3. Cell membrane; Single-pass type I membrane protein Ref.3.

Tissue specificity: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. Ref.1 Ref.2 Ref.3 Ref.12 Ref.16 Ref.19

Induction: Up-regulated in failing heart. Ref.1 Ref.2 Ref.3 Ref.13 Ref.15 Ref.19

Post-translational modification: N-glycosylation on Asn-90 may limit SARS infectivity.

Sequence similarities: Belongs to the peptidase M2 family.

Biophysicochemical propertiesKinetic parameters:KM=6.9 µM for angiotensin I Ref.13KM=2 µM for angiotensin IIKM=6.8 µM for apelin-13KM=5.5 µM for dynorphin-13pH dependence:Optimum pH is 6.5 in the presence of 1 M NaCl. Active from pH 6 to 9.