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Actin Beta (ACTb) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS726697

Intended Uses: This BACT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BACT. This ELISA kit for research use only, not for therapeutic or test applications!Principle of the Assay: BACT ELISA kit applies the… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Actin beta (ACTb), ELISA Kit
Full Product Name
Human Actin beta ELISA Kit
Product Gene Name
ACTb elisa kit
Product Synonym Gene Name
ACTb
Product Synonym Names
Human Actin b ELISA Kit
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of BACT. No significant cross-reactivity or interference between BACT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BACT and all the analogues, therefore, cross reaction may still exist in some cases.
Preparation and Storage
Store all reagents at 2-8 degree C.
Molecular Weight
41,737 Da
OMIM
607371
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Product Categories/Family for ACTb elisa kit

Cell Biology
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of ACTb elisa kit

Human ACTb ELISA Kit – Cat# MBS726697

Other Notes

Small volumes of ACTb elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTb purchase

MBS726697 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Actin beta (ACTb) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACTb. The ELISA analytical biochemical technique of the MBS726697 kit is based on ACTb antibody-ACTb antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACTb antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACTb. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ACTb. It may not necessarily be applicable to this product.

NCBI GI #
117167827
NCBI GeneID
60
NCBI Accession #
AAI13037.1
UniProt Secondary Accession #
P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
UniProt Related Accession #
P60709
NCBI Official Full Name
ACTB protein, partial
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actinCleaved into the following chain:Actin, cytoplasmic 1, N-terminally processed
Protein Family
Actin
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
NCBI Protein Information
actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1

NCBI Summary for ACTb

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

UniProt Comments for ACTb

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; protein complex; focal adhesion; postsynaptic density; cytosol; ribonucleoprotein complex; nucleoplasm; cytoskeleton; membrane; axon; cytoplasm; nuclear chromatin; plasma membrane

Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; kinesin binding; Tat protein binding; nitric-oxide synthase binding; protein kinase binding; ATP binding

Biological Process: intercellular junction assembly and maintenance; axon guidance; establishment and/or maintenance of chromatin architecture; protein folding; retinal homeostasis; 'de novo' posttranslational protein folding; cellular protein metabolic process; substantia nigra development; ATP-dependent chromatin remodeling; ephrin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; cell motility; blood coagulation

Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1
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Research Articles on ACTb

1. Taken together, these findings indicate that actin filament dynamics are dispensable for HIV-1 Gag assembly at the plasma membrane of HeLa cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ACTb elisa kit

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram

Diseases associated with ACTb elisa kit

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >47 publications with ACTb and Carcinoma
 Liver Diseases Antibodies  >23 publications with ACTb and Liver Diseases
 Brain Diseases Antibodies  >19 publications with ACTb and Brain Diseases
 Disease Models, Animal Antibodies  >18 publications with ACTb and Disease Models, Animal
 Lung Diseases Antibodies  >15 publications with ACTb and Lung Diseases
 Kidney Diseases Antibodies  >13 publications with ACTb and Kidney Diseases
 Necrosis Antibodies  >13 publications with ACTb and Necrosis
 Liver Neoplasms Antibodies  >12 publications with ACTb and Liver Neoplasms
 Heart Diseases Antibodies  >10 publications with ACTb and Heart Diseases
 Inflammation Antibodies  >10 publications with ACTb and Inflammation

Organs/Tissues associated with ACTb elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >66 publications with ACTb and Blood
 Liver Antibodies  >52 publications with ACTb and Liver
 Brain Antibodies  >51 publications with ACTb and Brain
 Muscle Antibodies  >49 publications with ACTb and Muscle
 Kidney Antibodies  >31 publications with ACTb and Kidney
 Bone Antibodies  >26 publications with ACTb and Bone
 Lung Antibodies  >25 publications with ACTb and Lung
 Heart Antibodies  >24 publications with ACTb and Heart
 Connective Tissue Antibodies  >20 publications with ACTb and Connective Tissue
 Embryonic Tissue Antibodies  >19 publications with ACTb and Embryonic Tissue
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