Complement Component 1 Q Receptor (C1qR1) ELISA Kit for Mouse

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This C1QR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C1QR. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY C1QR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C1QR antibody and an C1QR-HRP conjugate. The assay sample and buffer are incubated together with C1QR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C1QR concentration since C1QR from samples and C1QR-HRP conjugate compete for the anti-C1QR antibody binding site. Since the number of sites is limited, as more sites are occupied by C1QR from the sample, fewer sites are left to bind C1QR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1QR concentration in each sample is interpolated from this standard curve.

Small volumes of C1qR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS726892 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Component 1 Q Receptor (C1qR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C1qR1. The ELISA analytical biochemical technique of the MBS726892 kit is based on C1qR1 antibody-C1qR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C1qR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C1qR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).