MHC Class 1 Polypeptide Related Sequence B (MICB) ELISA Kit for Human

Name: Human MICB ELISA Kit; MHC Class 1 Polypeptide Related Sequence B; MyBioSource
Brand: MyBioSource
SKU: MBS726949
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS726949

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This MICB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MICB. This ELISA kit for research use only! PRINCIPLE… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

MHC class I polypeptide related sequence B (MICB), ELISA Kit

Full Product Name

Human MHC class I polypeptide related sequence B ELISA Kit

Product Gene Name

MICB elisa kit

Species Reactivity

Human

Assay Type

Competitive

Samples

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Detection Range

50-1000pg/mL

Sensitivity

1.0pg/mL

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

4,287 Da

OMIM

602436

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Product Categories/Family for MICB elisa kit

Immunology

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of MICB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MICB purchase

MBS726949 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MHC class I polypeptide related sequence B (MICB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MICB. The ELISA analytical biochemical technique of the MBS726949 kit is based on MICB antibody-MICB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MICB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MICB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MICB. It may not necessarily be applicable to this product.

NCBI GI #

167857788

NCBI GeneID

4277

NCBI Accession #

NP_005922.2

UniProt Secondary Accession #

O14499; O14500; O19798; O19799; O19800; O19801; O19802; A2AC57; A6NP85; B0UZ10; B2RAK2

UniProt Related Accession #

Q29980

GenBank Nucleotide #

NM_005931.4

NCBI Official Full Name

MHC class I polypeptide-related sequence B isoform 1

NCBI Official Synonym Full Names

MHC class I polypeptide-related sequence B

NCBI Official Symbol

MICB

NCBI Official Synonym Symbols

PERB11.2

UniProt Protein Name

MHC class I polypeptide-related sequence B

Protein Family

MHC class I polypeptide-related sequence

UniProt Gene Name

MICB

UniProt Synonym Gene Names

PERB11.2; MIC-B

UniProt Entry Name

MICB_HUMAN

NCBI Protein Information

MHC class I polypeptide-related sequence B; MHC class I mic-B antigen; stress inducible class I homolog; MHC class I chain-related protein B; MHC class I-like molecule PERB11.2-IMX

NCBI Summary for MICB

This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

UniProt Comments for MICB

MICB: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Genetic variations in MICA are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. The MICB*004 allele is associated with rheumatoid arthritis. Genetic variation in MICB is associated with cytomegalovirus and herpes simplex virus I seropositivity and this may be associated with schizophrenia risk. Belongs to the MHC class I family. MIC subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; integral to membrane; plasma membrane

Molecular Function: beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; regulation of immune response; response to retinoic acid; viral reproduction; natural killer cell mediated cytotoxicity; response to heat; cytolysis; immune response-activating cell surface receptor signaling pathway; response to oxidative stress; negative regulation of defense response to virus by host; gamma-delta T cell activation; T cell mediated cytotoxicity

Research Articles on MICB

1. Experiments demonstrate that infection in vitro with human cytomegalovirus leads to enhanced shedding of MICA and MICB.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with MICB elisa kit

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies	Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
Natural Killer Cell Mediated Cytotoxicity Pathway antibodies	Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
Natural Killer Cell Mediated Cytotoxicity Pathway antibodies	Natural Killer Cell Mediated Cytotoxicity Pathway Diagram

Diseases associated with MICB elisa kit

Disease Name	Pubmed Publications
Carcinoma Antibodies	>46 publications with MICB and Carcinoma
Adenocarcinoma Antibodies	>24 publications with MICB and Adenocarcinoma
Cardiovascular Diseases Antibodies	>20 publications with MICB and Cardiovascular Diseases
Necrosis Antibodies	>19 publications with MICB and Necrosis
Liver Diseases Antibodies	>17 publications with MICB and Liver Diseases
Liver Neoplasms Antibodies	>12 publications with MICB and Liver Neoplasms
Inflammation Antibodies	>10 publications with MICB and Inflammation
Pancreatic Neoplasms Antibodies	>10 publications with MICB and Pancreatic Neoplasms
Lung Diseases Antibodies	>10 publications with MICB and Lung Diseases
Neoplasm Metastasis Antibodies	>9 publications with MICB and Neoplasm Metastasis

Organs/Tissues associated with MICB elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>205 publications with MICB and Blood
Lung Antibodies	>18 publications with MICB and Lung
Liver Antibodies	>17 publications with MICB and Liver
Kidney Antibodies	>11 publications with MICB and Kidney
Intestine Antibodies	>10 publications with MICB and Intestine
Lymph Antibodies	>7 publications with MICB and Lymph
Bone Marrow Antibodies	>7 publications with MICB and Bone Marrow
Brain Antibodies	>5 publications with MICB and Brain
Vascular Antibodies	>3 publications with MICB and Vascular
Embryonic Tissue Antibodies	>3 publications with MICB and Embryonic Tissue