Growth Hormone Releasing Hormone (GHRH) ELISA Kit for Human

Name: Human GHRH ELISA Kit; Growth Hormone Releasing Hormone; MyBioSource
Brand: MyBioSource
SKU: MBS727174
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS727174

Principle of the Assay: GHRH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GHRH. Standards or samples are then added to the microtiter plate wells and… More details

$580 / 48-Strip-Wells-(Competitive) +1 FREE 8GB USB

Product Documentation

Sample Competitive Manual Insert View PDF

Sample Sandwich Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Growth hormone releasing hormone (GHRH), ELISA Kit

Full Product Name

Human Growth hormone releasing hormone ELISA Kit

Product Gene Name

GHRH elisa kit

Species Reactivity

Human

Assay Type

Quantitative Competitive or Sandwich

Samples

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Sensitivity

1.0pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of GHRH. No significant cross-reactivity or interference between GHRH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GHRH and all the analogues, therefore, cross reaction may still exist in some cases.

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

47,402 Da

OMIM

gene 612781

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of GHRH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GHRH purchase

MBS727174 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Growth hormone releasing hormone (GHRH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GHRH. The ELISA analytical biochemical technique of the MBS727174 kit is based on GHRH antibody-GHRH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GHRH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GHRH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GHRH. It may not necessarily be applicable to this product.

NCBI GI #

1706945

NCBI GeneID

2692

NCBI Accession #

AAB37758.1

UniProt Secondary Accession #

Q99863

UniProt Related Accession #

Q02643

NCBI Official Full Name

growth hormone releasing hormone, partial

NCBI Official Synonym Full Names

growth hormone releasing hormone receptor

NCBI Official Symbol

GHRHR

NCBI Official Synonym Symbols

GRFR; GHRFR; IGHD1B

UniProt Protein Name

Growth hormone-releasing hormone receptor

UniProt Synonym Protein Names

Growth hormone-releasing factor receptor; GRF receptor; GRFR

Protein Family

Somatoliberin

UniProt Gene Name

GHRHR

UniProt Synonym Gene Names

GHRH receptor; GRF receptor; GRFR

UniProt Entry Name

GHRHR_HUMAN

NCBI Protein Information

growth hormone-releasing hormone receptor; GRF receptor; GHRH receptor

NCBI Summary for GHRH

This gene encodes a receptor for growth hormone-releasing hormone. Binding of this hormone to the receptor leads to synthesis and release of growth hormone. Mutations in this gene have been associated with isolated growth hormone deficiency (IGHD), also known as Dwarfism of Sindh, a disorder characterized by short stature. [provided by RefSeq, Jun 2010]

UniProt Comments for GHRH

GHRHR: Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion. Defects in GHRHR are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Belongs to the G-protein coupled receptor 2 family.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 2; Cell development/differentiation

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: nuclear outer membrane; cell surface; nuclear matrix; cytoplasm; integral to membrane; plasma membrane; nuclear inner membrane; secretory granule

Molecular Function: G-protein coupled receptor activity; protein binding; peptide hormone binding; growth factor binding; growth hormone-releasing hormone receptor activity

Biological Process: lactation; positive regulation of insulin-like growth factor receptor signaling pathway; response to glucocorticoid stimulus; cell maturation; adenylate cyclase activation; regulation of protein metabolic process; determination of adult life span; positive regulation of multicellular organism growth; reproductive process in a multicellular organism; G-protein signaling, adenylate cyclase activating pathway; response to insulin stimulus; positive regulation of growth hormone secretion; water homeostasis; growth hormone secretion; cAMP-mediated signaling; cellular response to insulin stimulus; response to estrogen stimulus; somatotropin secreting cell development; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; hormone metabolic process; regulation of steroid hormone receptor signaling pathway

Disease: Isolated Growth Hormone Deficiency, Type Ib

Research Articles on GHRH

1. Compound heterozygous mutations of the growth hormone-releasing hormone receptor gene is associated with isolated growth hormone deficiency

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GHRH elisa kit

Reference Product	PubMed Publications
GHRH elisa kit	>56 publications with GHRH and GHRH

Pathways associated with GHRH elisa kit

Products by Pathway	Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class B Secretin-like Pathway antibodies	GPCRs, Class B Secretin-like Pathway Diagram
GPCRs, Other Pathway antibodies	GPCRs, Other Pathway Diagram
Glucagon-type Ligand Receptors Pathway antibodies	Glucagon-type Ligand Receptors Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram

Diseases associated with GHRH elisa kit

Disease Name	Pubmed Publications
Isolated Growth Hormone Deficiency, Type IB Antibodies	>11 publications with GHRH and Isolated Growth Hormone Deficiency, Type IB
Breast Neoplasms Antibodies	>4 publications with GHRH and Breast Neoplasms
Weight Loss Antibodies	>3 publications with GHRH and Weight Loss
Cardiovascular Diseases Antibodies	>2 publications with GHRH and Cardiovascular Diseases
Heart Diseases Antibodies	>2 publications with GHRH and Heart Diseases
Weight Gain Antibodies	>1 publications with GHRH and Weight Gain
Kidney Neoplasms Antibodies	>1 publications with GHRH and Kidney Neoplasms
Liver Diseases Antibodies	>1 publications with GHRH and Liver Diseases
Prostatic Diseases Antibodies	>1 publications with GHRH and Prostatic Diseases
Necrosis Antibodies	>1 publications with GHRH and Necrosis