Eosinophil Cationic (ECP) ELISA Kit for Human

Intended Uses: This ECP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ECP. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: ECP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ECP. Standards or samples are then added to the microtiter plate wells and ECP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ECP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ECP are added to each well to "sandwich" the ECP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ECP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ECP concentration in each sample is interpolated from this standard curve.

Small volumes of ECP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS727222 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Eosinophil cationic protein (ECP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ECP. The ELISA analytical biochemical technique of the MBS727222 kit is based on ECP antibody-ECP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ECP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ECP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).