Leukemia Inhibitory Factor (LIF) ELISA Kit for Human

Name: Human LIF ELISA Kit; Leukemia Inhibitory Factor; MyBioSource
Brand: MyBioSource
SKU: MBS727620
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS727620

Intended Uses: This LIF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LIF. This ELISA kit for research use only, not for therapeutic or test applications!Principle of the Assay: LIF ELISA kit applies the… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Leukemia Inhibitory Factor (LIF), ELISA Kit

Full Product Name

Human Leukemia Inhibitory Factor ELISA Kit

Product Gene Name

LIF elisa kit

Species Reactivity

Human

Assay Type

Quantitative Competitive

Samples

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Sensitivity

1.0 pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of LIF. No significant cross-reactivity or interference between LIF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LIF and all the analogues, therefore, cross reaction may still exist in some cases.

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

9,563 Da

OMIM

159540

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Product Categories/Family for LIF elisa kit

Immunology

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of LIF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIF purchase

MBS727620 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Leukemia Inhibitory Factor (LIF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LIF. The ELISA analytical biochemical technique of the MBS727620 kit is based on LIF antibody-LIF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LIF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LIF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LIF. It may not necessarily be applicable to this product.

NCBI GI #

4504991

NCBI GeneID

3976

NCBI Accession #

NP_002300.1

UniProt Secondary Accession #

Q52LZ2; B2RCW7; B5MC23

UniProt Related Accession #

P15018

GenBank Nucleotide #

NM_002309.4

NCBI Official Full Name

leukemia inhibitory factor isoform 1

NCBI Official Synonym Full Names

leukemia inhibitory factor

NCBI Official Symbol

LIF

NCBI Official Synonym Symbols

CDF; DIA; HILDA; MLPLI

UniProt Protein Name

Leukemia inhibitory factor

UniProt Synonym Protein Names

Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; INN: Emfilermin

Protein Family

Leukemia inhibitory factor

UniProt Gene Name

LIF

UniProt Synonym Gene Names

HILDA; LIF; D factor; MLPLI

UniProt Entry Name

LIF_HUMAN

NCBI Protein Information

leukemia inhibitory factor; D factor; human interleukin in DA cells; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; differentiation-stimulating factor; differentiation inhibitory activity

NCBI Summary for LIF

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

UniProt Comments for LIF

LIF: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. Belongs to the LIF/OSM family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: growth factor activity; leukemia inhibitory factor receptor binding; cytokine activity; receptor binding

Biological Process: transcription from RNA polymerase II promoter; multicellular organismal development; leukemia inhibitory factor signaling pathway; negative regulation of hormone secretion; tyrosine phosphorylation of Stat3 protein; decidualization; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; negative regulation of meiosis; positive regulation of macrophage differentiation; muscle morphogenesis; positive regulation of astrocyte differentiation; positive regulation of peptidyl-serine phosphorylation of STAT protein; stem cell maintenance; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat1 protein; neuron development; immune response; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; alveolus development; embryo implantation

Research Articles on LIF

1. Data from 3-year-old girl and other reported cases support model in which abundance of LIF in B-cell acute lymphoblastic leukemia results in leukemic infiltration of central nervous system and development of Cushing syndrome. [CASE STUDY; REVIEW]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LIF elisa kit

Reference Product	PubMed Publications
KITLG elisa kit	>1042 publications with LIF and KITLG
JUN elisa kit	>634 publications with LIF and JUN
STAT3 elisa kit	>548 publications with LIF and STAT3
LIFR elisa kit	>458 publications with LIF and LIFR
IL6ST elisa kit	>237 publications with LIF and IL6ST
NANOG elisa kit	>178 publications with LIF and NANOG
FOS elisa kit	>54 publications with LIF and FOS
DIF elisa kit	>9 publications with LIF and DIF
TP53 elisa kit	>5 publications with LIF and TP53

Pathways associated with LIF elisa kit

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
MicroRNAs In Cardiomyocyte Hypertrophy Pathway antibodies	MicroRNAs In Cardiomyocyte Hypertrophy Pathway Diagram
Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies	Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies	Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
TGF Beta Signaling Pathway antibodies	TGF Beta Signaling Pathway Diagram

Diseases associated with LIF elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>276 publications with LIF and Necrosis
Inflammation Antibodies	>271 publications with LIF and Inflammation
Cardiovascular Diseases Antibodies	>189 publications with LIF and Cardiovascular Diseases
Female Urogenital Diseases Antibodies	>159 publications with LIF and Female Urogenital Diseases
Infertility, Female Antibodies	>106 publications with LIF and Infertility, Female
Neoplasms, Experimental Antibodies	>85 publications with LIF and Neoplasms, Experimental
Heart Diseases Antibodies	>70 publications with LIF and Heart Diseases
Lung Diseases Antibodies	>65 publications with LIF and Lung Diseases
Liver Diseases Antibodies	>59 publications with LIF and Liver Diseases
Weight Loss Antibodies	>46 publications with LIF and Weight Loss

Organs/Tissues associated with LIF elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>503 publications with LIF and Bone
Brain Antibodies	>491 publications with LIF and Brain
Uterus Antibodies	>349 publications with LIF and Uterus
Embryonic Tissue Antibodies	>337 publications with LIF and Embryonic Tissue
Muscle Antibodies	>332 publications with LIF and Muscle
Bone Marrow Antibodies	>243 publications with LIF and Bone Marrow
Skin Antibodies	>182 publications with LIF and Skin
Lung Antibodies	>141 publications with LIF and Lung
Placenta Antibodies	>140 publications with LIF and Placenta
Eye Antibodies	>121 publications with LIF and Eye