Octamer Binding Transcription Factor 1 (OBTF1) ELISA Kit for Human

Name: Human OBTF1 ELISA Kit; Octamer Binding Transcription Factor 1; MyBioSource
Brand: MyBioSource
SKU: MBS729337
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS729337

Principle of the assay: OBTF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OBTF1 antibody and an OBTF1-HRP conjugate. The assay sample and buffer are incubated together with OBTF1-HRP conjugate in pre-coated plate… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Octamer Binding Transcription Factor 1 (OBTF1), ELISA Kit

Full Product Name

Human Octamer Binding Transcription Factor 1 ELISA Kit

Product Gene Name

OBTF1 elisa kit

Species Reactivity

Human

Assay Type

Competitive

Samples

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Detection Range

0.5-10ng/mL

Sensitivity

0.1ng/mL

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

42,697 Da

OMIM

601632

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Intended Uses

This OBTF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human OBTF1. This ELISA kit for research use only, not for therapeutic applications!

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of OBTF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OBTF1 purchase

MBS729337 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Octamer Binding Transcription Factor 1 (OBTF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OBTF1. The ELISA analytical biochemical technique of the MBS729337 kit is based on OBTF1 antibody-OBTF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OBTF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OBTF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for OBTF1. It may not necessarily be applicable to this product.

NCBI GI #

418016

NCBI Accession #

AAA65605.1

UniProt Secondary Accession #

Q14986; Q15318; Q5T227

NCBI Official Full Name

octamer binding transcription factor 1

UniProt Protein Name

POU domain, class 4, transcription factor 1

UniProt Synonym Protein Names

Brain-specific homeobox/POU domain protein 3A; Brain-3A; Brn-3A; Homeobox/POU domain protein RDC-1; Oct-T1

Protein Family

POU domain, class 2, transcription factor

UniProt Gene Name

POU4F1

UniProt Synonym Gene Names

BRN3A; RDC1; Brain-3A; Brn-3A

UniProt Entry Name

PO4F1_HUMAN

UniProt Comments for OBTF1

POU4F1: Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: DNA-binding; Transcription factor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 13q31.1

Cellular Component: neuron projection; nuclear chromatin

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding

Biological Process: transcription from RNA polymerase II promoter; trigeminal nerve development; central nervous system neuron differentiation; positive regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; habenula development; peripheral nervous system neuron development; cell migration in hindbrain; proprioception during equilibrioception; regulation of neurogenesis; synaptogenesis; suckling behavior; axonogenesis; neuron fate specification; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; sensory system development; negative regulation of neuron apoptosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.