Connexin 43 (CX43) ELISA Kit for Mouse

Principle of the Assay: CX43 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CX43 antibody and an CX43-HRP conjugate. The assay sample and buffer are incubated together with CX43-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX43 concentration since CX43 from samples and CX43-HRP conjugate compete for the anti-CX43 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX43 from the sample, fewer sites are left to bind CX43-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX43 concentration in each sample is interpolated from this standard curve.

Intended Uses: This CX43 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CX43. This ELISA kit for research use only, not for therapeutic or test applications!

Small volumes of CX43 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS729401 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Connexin 43 (CX43) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CX43. The ELISA analytical biochemical technique of the MBS729401 kit is based on CX43 antibody-CX43 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CX43 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CX43. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Intended use of this ELISA Kit is quantitative detection of murine connexin 43.

Kit’s target analyte, connexin 43, is one of major integral membrane proteins that form gap junction channels to provide inter-cellular communication such as exchange of small molecules and ions between adjacent cells. It’s expressed in variable cell types and plays important role in development, differentiation, and metabolic homeostasis.

Connexin 43 is listed in NCBI and UniProt data bases as gap junction alpha-1 protein, short fin protein; gap junction protein, and Cx43.

Murine connexin 43 test system is designed as sandwich-type ELISA and concentration of connexin 43 is measured using pair of antibodies specific to target protein.

Kit is applicable for testing connexin 43 in serum, plasma, cell culture supernatants, bodily fluids, and tissue homogenates.

Product contains microwell plate pre-coated with monoclonal capture antibodies against connexin 43 and all reagents required for its quantification, including sample and reagent diluents, and wash solution.

Assay kit provides colorimetric detection of connexin 43 using polyclonal detector antibodies against connexin 43 conjugated to HRP, and TMB as its substrate solution.

Minimal detectable concentration of ELISA kit is 1pg/mL; kits are supplied with six levels of liquid calibrators between 0 and 2500pg/mL.

Intra- and inter-lot assay CV values, reported as kit’s precision characteristics, are not greater than 9% and 10%, respectively.

Assay doesn’t show significant cross-reactivity with analogous molecules.

ELISA kit and its reagents and components require refrigeration and must be stored at 2-8°C until indicated expiration date.