Glomerular Basement Membrane (GBM) ELISA Kit for Human

Principle of the Assay: GBM ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GBM. Standards or samples are then added to the microtiter plate wells and GBM if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GBM present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for GBM are added to each well to "sandwich" the GBM immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GBM and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GBM concentration in each sample is interpolated from this standard curve.

Small volumes of GBM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS730640 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glomerular basement membrane antibody (GBM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GBM. The ELISA analytical biochemical technique of the MBS730640 kit is based on GBM antibody-GBM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GBM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GBM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).