Angiotensin 2 Receptor 1 Autoantibodies (AT1R-AA) ELISA Kit for Rat

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This AT1R-AA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat AT1R-AA. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY AT1R-AA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Angiotensin 2 Receptor 1 antigen. Standards or samples are then added to the microtiter plate wells and AT1R-AA if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of AT1R-AA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated Angiotensin 2 Receptor 1 antigen is added to each well to “sandwich” the AT1R-AA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AT1R-AA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT1R-AA concentration in each sample is interpolated from this standard curve.

Small volumes of AT1R-AA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS731165 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Angiotensin 2 Receptor 1 Autoantibodies (AT1R-AA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AT1R-AA. The ELISA analytical biochemical technique of the MBS731165 kit is based on AT1R-AA antibody-AT1R-AA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AT1R-AA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AT1R-AA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). The human angiotensin protein and receptor family are key blood pressure regulators. Autoantibodies IgG type, blockers of receptor signaling, are an endogenous BP reducing mechanism which is easily studied in rat model. Assay of these auto antibodies in cell or serum samples is accomplished with a specific angiotensin ii receptor (angiotensin 2 receptor ) 1 AT1R ELISA kit. Building quality anti 1 autoantibodies ELISA kits requires attention to details and specific monoclonal polyclonal products. Our assay receptor 1 autoantibodies IgG1 kits exceed your expectations, with anti antiotensin ii receptor type 1 antibody 96 tests plate or 48 wells strips, wash solution, standard curve product, all meeting the highest reagents products standards. We also offer related ELISA assay kit ankyrin or ELISA kit angiotensin or ELISA kit angiopoietin and a separate anti angiotensin ii antibody ELISA assay. With our current promotional pricing for assay kits, you can afford to repeat results.