Bone Alkaline Phosphatase (BALP) ELISA Kit for Mouse

Principle of the Assay: BALP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BALP antibody and an BALP-HRP conjugate. The assay sample and buffer are incubated together with BALP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BALP concentration since BALP from samples and BALP-HRP conjugate compete for the anti-BALP antibody binding site. Since the number of sites is limited, as more sites are occupied by BALP from the sample, fewer sites are left to bind BALP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BALP concentration in each sample is interpolated from this standard curve.

Intended Uses: This BALP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BALP. This ELISA kit for research use only, not for therapeutic or test applications!

Small volumes of BALP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS731438 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bone Alkaline Phosphatase (BALP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BALP. The ELISA analytical biochemical technique of the MBS731438 kit is based on BALP antibody-BALP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BALP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BALP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Bones are created, bones are destroyed. It's an ongoing struggle and as we age, the balance tips to loss. Research into the differentiation of bone cells and required bone morphogenetic protein require detection of bone alkaline phosphatase. Mouse bone alkaline phosphatase BALP is a biomarker for the differentiation in cell culture models of human osteoporosis. For the greatest sensitivity and dynamic range, we recommend our BALP ELISA kit. With ng ml sample precision, prebound assay plate, sample diluent, secondary antibodies, standard protein and rapid performance, our ELISA kit from CUSABio will meet your needs. We have samples concentration tested twenty times to validate assay precision. These ELISA kits are ideal for your small molecule screening assays and meet CLIA kits standards for molecular biology reagents. Full application details and potential target details come with kit product. We also offer BALP ELISA kit human and ELISA kit rat. Order today!