Anti-P62 (P62) ELISA Kit for Mouse

Intended Uses: This Anit-P62 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Anit-P62. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: Anit-P62 ELISA kit applies the competitive enzyme immunoassay technique utilizing -P62 antigen and an Anit-P62-HRP conjugate. The assay sample and buffer are incubated together with Anit-P62-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anit-P62 concentration since Anit-P62 from samples and Anit-P62-HRP conjugate compete for the -P62 antigen binding site. Since the number of sites is limited, as more sites are occupied by Anit-P62 from the sample, fewer sites are left to bind Anit-P62-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anit-P62 concentration in each sample is interpolated from this standard curve.

Small volumes of P62 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS732589 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-P62 Antibody, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing P62. The ELISA analytical biochemical technique of the MBS732589 kit is based on P62 antibody-P62 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect P62 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, P62. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).