Protein Z Dependent Protease Inhibitor (ZPI) ELISA Kit for Human

Principle of the Assay: ZPI ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ZPI. Standards or samples are then added to the microtiter plate wells and ZPI if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ZPI present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ZPI are added to each well to "sandwich" the ZPI immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ZPI and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZPI concentration in each sample is interpolated from this standard curve.

Small volumes of ZPI elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS733815 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Z Dependent Protease Inhibitor (ZPI) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZPI. The ELISA analytical biochemical technique of the MBS733815 kit is based on ZPI antibody-ZPI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZPI antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZPI. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). In the alphabet of proteins associated with the coagulation cascade, Protein Z acts as the end termination point to inhibit upstream serine proteases by accelerating ZPI enzymatic activity. Detection of Protein Z dependent protease inhibitor ZPI levels in human plasma or liver samples is possible with a Cloud Clone Corp ZPI Elisa kit. Each protein dependent protease enzyme assay kit we offer is the same quality precision product, complete with a precoated plate of mus musculus mouse antibody against homo sapiens human inhibitor ZPI, recombinant protein for standard curve generation, HRP conjugated sec antibodies for high sensitivity, excellent detection range (below 60ng ml), ease of performance and enzymatic MBS colorimetric visualization. Available in 2 economical quantities, a 48 well or 96 strip plate, for any lab budget. When comparing competing ELISA kits to our Clone Corp product for life science research, our value and commitment to customer needs stands out.