Melanoma Associated Antigen 1 (MAA1) ELISA Kit for Human

For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Intended Uses: This MAGE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MAGE1. This ELISA kit for research use only!

Principle of the Assay: MAGE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MAGE1 antibody and an MAGE1-HRP conjugate. The assay sample and buffer are incubated together with MAGE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAGE1 concentration since MAGE1 from samples and MAGE1-HRP conjugate compete for the anti-MAGE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MAGE1 from the sample, fewer sites are left to bind MAGE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAGE1 concentration in each sample is interpolated from this standard curve.

Small volumes of MAA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS733931 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Melanoma associated antigen 1 (MAA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MAA1. The ELISA analytical biochemical technique of the MBS733931 kit is based on MAA1 antibody-MAA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MAA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MAA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).