Crystal Proteins Beta (CRYBETA) ELISA Kit for Canine

Principle of the Assay: Crybeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Crybeta antibody and an Crybeta-HRP conjugate. The assay sample and buffer are incubated together with Crybeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Crybeta concentration since Crybeta from samples and Crybeta-HRP conjugate compete for the anti-Crybeta antibody binding site. Since the number of sites is limited, as more sites are occupied by Crybeta from the sample, fewer sites are left to bind Crybeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Crybeta concentration in each sample is interpolated from this standard curve.

Small volumes of Crybeta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS734326 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Crystal proteins beta (Crybeta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Crybeta. The ELISA analytical biochemical technique of the MBS734326 kit is based on Crybeta antibody-Crybeta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Crybeta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Crybeta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).