Anti-Neuronal Nuclear Autoantibody 1 (ANNA1) ELISA Kit for Canine

Name: Canine ANNA1 ELISA Kit; Anti-Neuronal Nuclear Autoantibody 1; MyBioSource
Brand: MyBioSource
SKU: MBS735831
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS735831

Principle of the Assay: ANNA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ANNA1 antibody and an ANNA1-HRP conjugate. The assay sample and buffer are incubated together with ANNA1-HRP conjugate in pre-coated plate… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Anti-Neuronal Nuclear AutoAntibody 1 (ANNA1), ELISA Kit

Full Product Name

Canine Anti-Neuronal Nuclear AutoAntibody 1 ELISA Kit

Product Gene Name

ANNA1 elisa kit

Species Reactivity

Canine

Assay Type

Quantitative Competitive

Samples

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Sensitivity

1.0 ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of ANNA1. No significant cross-reactivity or interference between ANNA1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANNA1 and all the analogues, therefore, cross reaction may still exist in some cases.

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

38,829 Da

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Product Categories/Family for ANNA1 elisa kit

Neurobiology

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of ANNA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ANNA1 purchase

MBS735831 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Neuronal Nuclear AutoAntibody 1 (ANNA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANNA1. The ELISA analytical biochemical technique of the MBS735831 kit is based on ANNA1 antibody-ANNA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANNA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANNA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ANNA1. It may not necessarily be applicable to this product.

NCBI GI #

6978501

NCBI GeneID

25380

NCBI Accession #

NP_037036.1

UniProt Secondary Accession #

Q64664

UniProt Related Accession #

P07150

GenBank Nucleotide #

NM_012904.2

NCBI Official Full Name

annexin A1

NCBI Official Synonym Full Names

annexin A1

NCBI Official Symbol

Anxa1

NCBI Official Synonym Symbols

p35; Anx1

UniProt Protein Name

Annexin A1

UniProt Synonym Protein Names

Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35

UniProt Gene Name

Anxa1

UniProt Synonym Gene Names

Anx1

UniProt Entry Name

ANXA1_RAT

NCBI Protein Information

annexin A1; annexin I; annexin-1; calpactin-2; calpactin II; lipocortin 1; lipocortin I; chromobindin-9; Annexin 1 (p35) (Lipocortin 1); phospholipase A2 inhibitory protein

NCBI Summary for ANNA1

plays a role in regulation of insulin secretion; may inhibit phopsholipase A2 [RGD, Feb 2006]

UniProt Comments for ANNA1

ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding

Cellular Component: extracellular space; focal adhesion; protein complex; cell surface; mast cell granule; basolateral plasma membrane; cornified envelope; cilium; cytoplasm; mitochondrial membrane; plasma membrane; nucleus; sarcolemma; endosome; vesicle

Molecular Function: protein binding, bridging; protein homodimerization activity; single-stranded RNA binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; double-stranded DNA-dependent ATPase activity; phospholipid binding; calcium ion binding; structural molecule activity; helicase activity; calcium-dependent protein binding; single-stranded DNA binding

Biological Process: prostate gland development; response to peptide hormone stimulus; positive regulation of apoptosis; response to glucocorticoid stimulus; response to hormone stimulus; positive regulation of vesicle fusion; alpha-beta T cell differentiation; signal transduction; response to organic cyclic substance; response to estradiol stimulus; response to corticosteroid stimulus; cell surface receptor linked signal transduction; positive regulation of prostaglandin biosynthetic process; response to X-ray; response to drug; negative regulation of protein secretion; positive regulation of neutrophil apoptosis; gliogenesis; neutrophil homeostasis; arachidonic acid secretion; endocrine pancreas development; DNA duplex unwinding; regulation of cell proliferation; keratinocyte differentiation; insulin secretion; DNA strand renaturation; peptide cross-linking

Research Articles on ANNA1

1. ANX A1 down-regulation of PMVEC proliferation in the presence of HPS-rat-serum may be the major cause of aberrant dysregulation of cytoskeletal proteins

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ANNA1 elisa kit

Reference Product	PubMed Publications
Egfr elisa kit	>5 publications with ANNA1 and Egfr
S100a11 elisa kit	>5 publications with ANNA1 and S100a11
S100a6 elisa kit	>2 publications with ANNA1 and S100a6

Pathways associated with ANNA1 elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway antibodies	Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Prostaglandin Synthesis And Regulation Pathway antibodies	Prostaglandin Synthesis And Regulation Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram

Diseases associated with ANNA1 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>75 publications with ANNA1 and Inflammation
Carcinoma Antibodies	>53 publications with ANNA1 and Carcinoma
Disease Models, Animal Antibodies	>24 publications with ANNA1 and Disease Models, Animal
Neoplasm Metastasis Antibodies	>18 publications with ANNA1 and Neoplasm Metastasis
Brain Diseases Antibodies	>18 publications with ANNA1 and Brain Diseases
Lung Diseases Antibodies	>16 publications with ANNA1 and Lung Diseases
Carcinoma, Squamous Cell Antibodies	>15 publications with ANNA1 and Carcinoma, Squamous Cell
Necrosis Antibodies	>12 publications with ANNA1 and Necrosis
Cardiovascular Diseases Antibodies	>11 publications with ANNA1 and Cardiovascular Diseases
Pain Antibodies	>9 publications with ANNA1 and Pain

Organs/Tissues associated with ANNA1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>29 publications with ANNA1 and Brain
Lung Antibodies	>22 publications with ANNA1 and Lung
Liver Antibodies	>15 publications with ANNA1 and Liver
Pituitary Gland Antibodies	>13 publications with ANNA1 and Pituitary Gland
Stomach Antibodies	>10 publications with ANNA1 and Stomach
Muscle Antibodies	>10 publications with ANNA1 and Muscle
Kidney Antibodies	>8 publications with ANNA1 and Kidney
Prostate Antibodies	>7 publications with ANNA1 and Prostate
Adipose Tissue Antibodies	>7 publications with ANNA1 and Adipose Tissue
Eye Antibodies	>6 publications with ANNA1 and Eye