Kallikrein 11 (KLK11) ELISA Kit for Chicken

Principle of the Assay: KLK11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KLK11 antibody and an KLK11-HRP conjugate. The assay sample and buffer are incubated together with KLK11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLK11 concentration since KLK11 from samples and KLK11-HRP conjugate compete for the anti-KLK11 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLK11 from the sample, fewer sites are left to bind KLK11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLK11 concentration in each sample is interpolated from this standard curve. 3. MATERIALS All reagents provided are stored at 2-8 degree C. Refer to the expiration date on the label. FOR RESEARCH USE ONLY.

Small volumes of KLK11 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS736245 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kallikrein 11 (KLK11) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KLK11. The ELISA analytical biochemical technique of the MBS736245 kit is based on KLK11 antibody-KLK11 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLK11 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLK11. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).