Small Nuclear Ribonucleoprotein Polypeptide C (SNRPC) ELISA Kit for Chicken

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This SNRPC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken SNRPC. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY SNRPC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SNRPC antibody and an SNRPC-HRP conjugate. The assay sample and buffer are incubated together with SNRPC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SNRPC concentration since SNRPC from samples and SNRPC-HRP conjugate compete for the anti-SNRPC antibody binding site. Since the number of sites is limited, as more sites are occupied by SNRPC from the sample, fewer sites are left to bind SNRPC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNRPC concentration in each sample is interpolated from this standard curve.

Small volumes of SNRPC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS738079 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Small Nuclear Ribonucleoprotein Polypeptide C (SNRPC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SNRPC. The ELISA analytical biochemical technique of the MBS738079 kit is based on SNRPC antibody-SNRPC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SNRPC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SNRPC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).