Allograft Inflammatory Factor 1 (AIF1) ELISA Kit for Porcine

Name: Porcine AIF1 ELISA Kit; Allograft Inflammatory Factor 1; MyBioSource
Brand: MyBioSource
SKU: MBS738180
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS738180

Principle of the Assay: AIF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AIF1 antibody and an AIF1-HRP conjugate. The assay sample and buffer are incubated together with AIF1-HRP conjugate in pre-coated plate for… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Allograft Inflammatory Factor 1 (AIF1), ELISA Kit

Full Product Name

Porcine Allograft Inflammatory Factor 1 ELISA Kit

Product Gene Name

AIF1 elisa kit

Species Reactivity

Porcine

Assay Type

Competitive

Samples

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Sensitivity

0.1 ng/mL.

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

14,748 Da

OMIM

601833

3D Structure

ModBase 3D Structure for Q9UIV4

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Intended Uses

This AIF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine AIF1. This ELISA kit for research use only, not for therapeutic applications

Product Categories/Family for AIF1 elisa kit

Immunology

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of AIF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AIF1 purchase

MBS738180 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Allograft Inflammatory Factor 1 (AIF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AIF1. The ELISA analytical biochemical technique of the MBS738180 kit is based on AIF1 antibody-AIF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AIF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AIF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AIF1. It may not necessarily be applicable to this product.

NCBI GI #

49457302

NCBI GeneID

199

NCBI Accession #

CAG46950.1

UniProt Primary Accession #

Q9UIV4

UniProt Secondary Accession #

O43904; Q9UIV4; Q9UKS9; A8K406

UniProt Related Accession #

P55008

NCBI Official Full Name

AIF1, partial

NCBI Official Synonym Full Names

allograft inflammatory factor 1

NCBI Official Symbol

AIF1

NCBI Official Synonym Symbols

IBA1; IRT1; AIF-1; IRT-1

UniProt Protein Name

Allograft inflammatory factor 1

UniProt Synonym Protein Names

Ionized calcium-binding adapter molecule 1; Protein G1

Protein Family

Allograft inflammatory factor

UniProt Gene Name

AIF1

UniProt Synonym Gene Names

G1; IBA1; AIF-1

UniProt Entry Name

AIF1_HUMAN

NCBI Protein Information

allograft inflammatory factor 1; protein G1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; allograft inflammatory factor-1 splice variant Hara-1

NCBI Summary for AIF1

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

UniProt Comments for AIF1

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: lamellipodium; perinuclear region of cytoplasm; cytoplasm; actin filament; perikaryon; nucleus; cytosol; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; microglial cell activation; Rac protein signal transduction; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; response to axon injury; positive regulation of protein amino acid phosphorylation; phagocytosis, engulfment; inflammatory response; response to electrical stimulus; negative regulation of apoptosis

Research Articles on AIF1

1. AIF-1 functions as a tumor suppressor possibly by regulating beta-catenin in gastric cancer. the level of AIF-1 expression may serve as a protective prognostic indicator for gastric cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AIF1 elisa kit

Reference Product	PubMed Publications
GFAP elisa kit	>87 publications with AIF1 and GFAP
RAC1 elisa kit	>4 publications with AIF1 and RAC1
TYROBP elisa kit	>1 publications with AIF1 and TYROBP

Pathways associated with AIF1 elisa kit

Products by Pathway	Pathway Diagram
Spinal Cord Injury Pathway antibodies	Spinal Cord Injury Pathway Diagram

Diseases associated with AIF1 elisa kit

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>257 publications with AIF1 and Disease Models, Animal
Inflammation Antibodies	>141 publications with AIF1 and Inflammation
Cardiovascular Diseases Antibodies	>95 publications with AIF1 and Cardiovascular Diseases
Necrosis Antibodies	>86 publications with AIF1 and Necrosis
Nerve Degeneration Antibodies	>41 publications with AIF1 and Nerve Degeneration
Poisoning Antibodies	>18 publications with AIF1 and Poisoning
Neurotoxicity Syndromes Antibodies	>16 publications with AIF1 and Neurotoxicity Syndromes
Cognition Disorders Antibodies	>15 publications with AIF1 and Cognition Disorders
Edema Antibodies	>12 publications with AIF1 and Edema
Learning Disorders Antibodies	>11 publications with AIF1 and Learning Disorders

Organs/Tissues associated with AIF1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>385 publications with AIF1 and Brain
Blood Antibodies	>130 publications with AIF1 and Blood
Vascular Antibodies	>57 publications with AIF1 and Vascular
Muscle Antibodies	>47 publications with AIF1 and Muscle
Bone Marrow Antibodies	>38 publications with AIF1 and Bone Marrow
Eye Antibodies	>27 publications with AIF1 and Eye
Embryonic Tissue Antibodies	>18 publications with AIF1 and Embryonic Tissue
Heart Antibodies	>16 publications with AIF1 and Heart
Skin Antibodies	>14 publications with AIF1 and Skin
Liver Antibodies	>14 publications with AIF1 and Liver