Hemopexin (HPX) ELISA Kit for Chicken

Principle of the Assay: HPX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HPX antibody and an HPX-HRP conjugate. The assay sample and buffer are incubated together with HPX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HPX concentration since HPX from samples and HPX-HRP conjugate compete for the anti-HPX antibody binding site. Since the number of sites is limited, as more sites are occupied by HPX from the sample, fewer sites are left to bind HPX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HPX concentration in each sample is interpolated from this standard curve. FOR RESEARCH USE ONLY.

Small volumes of HPX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS738296 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hemopexin (HPX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HPX. The ELISA analytical biochemical technique of the MBS738296 kit is based on HPX antibody-HPX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HPX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HPX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Low serum or tissue iron levels can result from dysfunctions in signal transduction pathways affecting gene regulation of iron scavenging proteins such as hemopexin hpx or improper recycling due to a loss of heparan sulfate binding of human hemopexin on heptacytes. In chickens, this plasma protein can be induced with stress and increased expression is indicative of inflammatory conditions. Thus cellular biochemical assays, such as the competitive chicken hemopexin elisa kit, utilizing specific primary antibodies and HRP secondary antibodies are an important development. These kits are complete with all required kits reagents including protein standards to create a standard curve. Further, these kits do not require extensive sample purification concentration kits prior to incubation and compare favorably to human hemopexin elisa kits in ng ml sensitivities. Minimal cross-reactivity is seen in mouse, rabbit or rat. Kits are shipped within 13 days and available in multiple formats. Inquire about 96 tests.