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Aquaporin 1 (AQP-1) ELISA Kit for Canine

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS738608

Principle of the Assay: AQP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AQP1 antibody and an AQP1-HRP conjugate. The assay sample and buffer are incubated together with AQP1-HRP conjugate in pre-coated plate for… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Aquaporin 1 (AQP-1), ELISA Kit
Full Product Name
Canine Aquaporin 1 ELISA Kit
Product Gene Name
AQP-1 elisa kit
Species Reactivity
Canine
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of AQP1. No significant cross-reactivity or interference between AQP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AQP1 and all the analogues, therefore, cross reaction may still exist in some cases.
Preparation and Storage
Store all reagents at 2-8 degree C.
Molecular Weight
28,857 Da
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Product Categories/Family for AQP-1 elisa kit

Signal Transduction
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of AQP-1 elisa kit

Canine AQP-1 ELISA Kit – Cat# MBS738608

Other Notes

Small volumes of AQP-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AQP-1 purchase

MBS738608 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aquaporin 1 (AQP-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AQP-1. The ELISA analytical biochemical technique of the MBS738608 kit is based on AQP-1 antibody-AQP-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AQP-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AQP-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AQP-1. It may not necessarily be applicable to this product.

NCBI GI #
6978527
NCBI GeneID
25240
NCBI Accession #
NP_036910.1
UniProt Secondary Accession #
Q5EB50; Q7TN36
UniProt Related Accession #
P29975
GenBank Nucleotide #
NM_012778.1
NCBI Official Full Name
aquaporin-1
NCBI Official Synonym Full Names
aquaporin 1
NCBI Official Symbol
Aqp1
NCBI Official Synonym Symbols
CHIP28
UniProt Protein Name
Aquaporin-1
UniProt Synonym Protein Names
Aquaporin-CHIP; Water channel protein for red blood cells and kidney proximal tubule
Protein Family
Aquaporin
UniProt Gene Name
Aqp1
UniProt Synonym Gene Names
Chip28; AQP-1
UniProt Entry Name
AQP1_RAT
NCBI Protein Information
aquaporin-1; AQP-1; aquaporin-CHIP; Aquaporin 1 (aquaporin channel forming integral protein 28 (CHIP)); water channel protein for red blood cells and kidney proximal tubule

NCBI Summary for AQP-1

integral membrane protein that is a major water transport molecule in the kidney proximal tubule and red blood cells [RGD, Feb 2006]

UniProt Comments for AQP-1

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Membrane protein, multi-pass; Transporter, aquaporin family; Membrane protein, integral; Transporter

Cellular Component: neuron projection; nuclear membrane; cell; brush border membrane; basolateral plasma membrane; integral to plasma membrane; integral to membrane; membrane; axon; apical part of cell; apical plasma membrane; cytoplasm; basal plasma membrane; plasma membrane; nerve terminal; nucleus; sarcolemma; brush border

Molecular Function: protein binding; potassium channel activity; ephrin receptor binding; water transporter activity; intracellular cGMP activated cation channel activity; ammonium transmembrane transporter activity; potassium ion transmembrane transporter activity; glycerol transmembrane transporter activity; water channel activity; nitric oxide transporter activity; transmembrane transporter activity; glycerol channel activity

Biological Process: glycerol transport; cGMP biosynthetic process; cerebrospinal fluid secretion; wound healing; water transport; carbon dioxide transport; response to hormone stimulus; negative regulation of caspase activity; pancreatic juice secretion; sensory perception of pain; positive regulation of saliva secretion; odontogenesis; water homeostasis; positive regulation of fibroblast proliferation; nitric oxide transport; hyperosmotic salinity response; multicellular organismal water homeostasis; cellular homeostasis; lipid digestion; potassium ion transport; lateral ventricle development; secretion by cell; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; hyperosmotic response; renal water transport; positive regulation of angiogenesis; camera-type eye morphogenesis; response to estrogen stimulus; adrenocorticotropin hormone secretion; establishment and/or maintenance of actin cytoskeleton polarity; cell volume homeostasis; positive regulation of cell migration; negative regulation of apoptosis
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Research Articles on AQP-1

1. Aqp1 enhances Bone marrow mesenchymal stem cell migration ability mainly through the focal adhesion kinase pathway and partially through the beta-catenin pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with AQP-1 elisa kit

 Products by Pathway  Pathway Diagram
 Aquaporin-mediated Transport Pathway antibodies  Aquaporin-mediated Transport Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway antibodies  Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway Diagram
 Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway antibodies  Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 O2/CO2 Exchange In Erythrocytes Pathway antibodies  O2/CO2 Exchange In Erythrocytes Pathway Diagram
 Passive Transport By Aquaporins Pathway antibodies  Passive Transport By Aquaporins Pathway Diagram
 Proximal Tubule Bicarbonate Reclamation Pathway antibodies  Proximal Tubule Bicarbonate Reclamation Pathway Diagram
 Proximal Tubule Bicarbonate Reclamation Pathway antibodies  Proximal Tubule Bicarbonate Reclamation Pathway Diagram

Diseases associated with AQP-1 elisa kit

 Disease Name  Pubmed Publications
 Edema Antibodies  >157 publications with AQP-1 and Edema
 Kidney Diseases Antibodies  >125 publications with AQP-1 and Kidney Diseases
 Nervous System Diseases Antibodies  >100 publications with AQP-1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >97 publications with AQP-1 and Disease Models, Animal
 Lung Diseases Antibodies  >53 publications with AQP-1 and Lung Diseases
 Carcinoma Antibodies  >47 publications with AQP-1 and Carcinoma
 Pulmonary Edema Antibodies  >40 publications with AQP-1 and Pulmonary Edema
 Inflammation Antibodies  >36 publications with AQP-1 and Inflammation
 Fibrosis Antibodies  >36 publications with AQP-1 and Fibrosis
 Hypertension Antibodies  >28 publications with AQP-1 and Hypertension

Organs/Tissues associated with AQP-1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >384 publications with AQP-1 and Kidney
 Brain Antibodies  >197 publications with AQP-1 and Brain
 Lung Antibodies  >149 publications with AQP-1 and Lung
 Eye Antibodies  >139 publications with AQP-1 and Eye
 Heart Antibodies  >56 publications with AQP-1 and Heart
 Artery Antibodies  >42 publications with AQP-1 and Artery
 Embryonic Tissue Antibodies  >35 publications with AQP-1 and Embryonic Tissue
 Placenta Antibodies  >18 publications with AQP-1 and Placenta
 Connective Tissue Antibodies  >17 publications with AQP-1 and Connective Tissue
 Ovary Antibodies  >15 publications with AQP-1 and Ovary
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