Procollagen Type 1 C-Terminal Pro (PICP) ELISA Kit for Chicken

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This PICP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken PICP. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY PICP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PICP antibody and an PICP-HRP conjugate. The assay sample and buffer are incubated together with PICP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PICP concentration since PICP from samples and PICP-HRP conjugate compete for the anti-PICP antibody binding site. Since the number of sites is limited, as more sites are occupied by PICP from the sample, fewer sites are left to bind PICP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PICP concentration in each sample is interpolated from this standard curve.

Small volumes of PICP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS738748 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Procollagen Type I C-Terminal Propeptide (PICP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PICP. The ELISA analytical biochemical technique of the MBS738748 kit is based on PICP antibody-PICP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PICP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PICP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Tissues require physical structure and extracellular matrix works to both maintain integrity and promotes intercellular communication. Full length native protein is the result of cleavage of procollagen iii in serum plasma in homo sapiens human. Chickens also express typical procollagen terminal propeptide products. Quantitation of procollagen iii terminal propeptide PICP is possible with a precision ELISA kit pro collagen terminal propeptide PINP (aka terminal propeptide PIIINP) from MyBiosource. Our ELISA kits are designed to efficiently determine samples middle high level procollagen expression and are ideal for serum plasma biological fluids analysis with precision intra assay and inter assay precision. Easy to perform, with specificity assay high due to our quality components, including 48 wells strips or 96 well plate, primary antibody, secondary antibodies, standard curve reagent for sample read comparison, diluent, wash and stop solution, and detection reagent. Pair this product to reagents like ELISA kit carboxypeptidase for complete analyses.