Fatty Acid Synthase (FAS) ELISA Kit for Chicken

Name: Chicken FAS ELISA Kit; Fatty Acid Synthase; MyBioSource
Brand: MyBioSource
SKU: MBS739474
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS739474

Principle of the Assay: FASN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FASN antibody and an FASN-HRP conjugate. The assay sample and buffer are incubated together with FASN-HRP conjugate in pre-coated plate for… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Fatty Acid Synthase (FAS), ELISA Kit

Full Product Name

Chicken Fatty Acid Synthase ELISA Kit

Product Gene Name

FAS elisa kit

Species Reactivity

Chicken

Assay Type

Quantitative Competitive

Samples

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Sensitivity

1.0 pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of FASN. No significant cross-reactivity or interference between FASN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FASN and all the analogues, therefore, cross reaction may still exist in some cases.

Preparation and Storage

Store all reagents at 2-8 degree C

Molecular Weight

273,427 Da

Chromosome Location

Chromosome: 17; NC_000017.10 (80036214..80056106, complement). Location: 17q25

OMIM

600212

3D Structure

ModBase 3D Structure for P49327

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Product Categories/Family for FAS elisa kit

Cardiovascular

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of FAS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FAS purchase

MBS739474 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fatty Acid Synthase (FAS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FAS. The ELISA analytical biochemical technique of the MBS739474 kit is based on FAS antibody-FAS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FAS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FAS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FAS. It may not necessarily be applicable to this product.

NCBI GI #

41872631

NCBI GeneID

2194

NCBI Accession #

NP_004095.4

UniProt Primary Accession #

P49327

UniProt Secondary Accession #

Q13479; Q16702; Q4LE83; Q6P4U5; Q6SS02; Q969R1; Q96C68; Q96IT0

UniProt Related Accession #

P49327; Q13587; Q6PJJ3

GenBank Nucleotide #

NM_004104.4

NCBI Official Full Name

fatty acid synthase

NCBI Official Synonym Full Names

fatty acid synthase

NCBI Official Symbol

FASN

NCBI Official Synonym Symbols

FAS; OA-519; SDR27X1

UniProt Protein Name

Fatty acid synthase

Protein Family

Tumor necrosis factor receptor superfamily

UniProt Gene Name

FASN

UniProt Synonym Gene Names

FAS

UniProt Entry Name

FAS_HUMAN

NCBI Protein Information

fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1

NCBI Summary for FAS

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

UniProt Comments for FAS

FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Protein type: Acetyltransferase; EC 4.2.1.59; EC 1.1.1.100; EC 2.3.1.85; Lipid Metabolism - fatty acid biosynthesis; EC 2.3.1.38; EC 1.3.1.39; Hydrolase; EC 3.1.2.14; Oxidoreductase; EC 2.3.1.39; Lyase; EC 2.3.1.41

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: Golgi apparatus; membrane; mitochondrion; glycogen granule; cytoplasm; melanosome; plasma membrane; cytosol

Molecular Function: fatty-acid synthase activity; protein homodimerization activity; myristoyl-[acyl-carrier-protein] hydrolase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; zinc ion binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; drug binding; [acyl-carrier-protein] S-acetyltransferase activity; 3-oxoacyl-[acyl-carrier-protein] reductase activity; protein binding; 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; [acyl-carrier-protein] S-malonyltransferase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity

Biological Process: osteoblast differentiation; vitamin metabolic process; acetyl-CoA metabolic process; pantothenate metabolic process; energy reserve metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid metabolic process; water-soluble vitamin metabolic process; fatty acid biosynthetic process

Research Articles on FAS

1. FASN inhibition not only abrogates lipogenesis, which is indispensable for cancer growth, but also downregulates oncogenic PI3K signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with FAS elisa kit

Products by Pathway	Pathway Diagram
ChREBP Activates Metabolic Gene Expression Pathway antibodies	ChREBP Activates Metabolic Gene Expression Pathway Diagram
Fatty Acid Biosynthesis Pathway antibodies	Fatty Acid Biosynthesis Pathway Diagram
Fatty Acyl-CoA Biosynthesis Pathway antibodies	Fatty Acyl-CoA Biosynthesis Pathway Diagram
Fatty Acid Biosynthesis Pathway antibodies	Fatty Acid Biosynthesis Pathway Diagram
Fatty Acid Biosynthesis Pathway antibodies	Fatty Acid Biosynthesis Pathway Diagram
Fatty Acid Biosynthesis, Elongation Pathway antibodies	Fatty Acid Biosynthesis, Elongation Pathway Diagram
Fatty Acid Biosynthesis, Elongation Pathway antibodies	Fatty Acid Biosynthesis, Elongation Pathway Diagram
Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies	Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram

Diseases associated with FAS elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>119 publications with FAS and Neoplasms
Fatty Liver Antibodies	>63 publications with FAS and Fatty Liver
Obesity Antibodies	>52 publications with FAS and Obesity
Breast Neoplasms Antibodies	>31 publications with FAS and Breast Neoplasms
Liver Diseases Antibodies	>26 publications with FAS and Liver Diseases
Insulin Resistance Antibodies	>25 publications with FAS and Insulin Resistance
Gastrointestinal Diseases Antibodies	>21 publications with FAS and Gastrointestinal Diseases
Weight Loss Antibodies	>15 publications with FAS and Weight Loss
Inflammation Antibodies	>14 publications with FAS and Inflammation
Disease Models, Animal Antibodies	>13 publications with FAS and Disease Models, Animal

Organs/Tissues associated with FAS elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>72 publications with FAS and Liver
Adipose Tissue Antibodies	>51 publications with FAS and Adipose Tissue
Blood Antibodies	>47 publications with FAS and Blood
Connective Tissue Antibodies	>32 publications with FAS and Connective Tissue
Prostate Antibodies	>28 publications with FAS and Prostate
Muscle Antibodies	>26 publications with FAS and Muscle
Mammary Gland Antibodies	>12 publications with FAS and Mammary Gland
Brain Antibodies	>11 publications with FAS and Brain
Kidney Antibodies	>7 publications with FAS and Kidney
Intestine Antibodies	>6 publications with FAS and Intestine