Luteinizing Hormone-Releasing Hormone (LHRH) ELISA Kit for Chicken

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This LHRH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken LHRH. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY LHRH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LHRH antibody and an LHRH-HRP conjugate. The assay sample and buffer are incubated together with LHRH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LHRH concentration since LHRH from samples and LHRH-HRP conjugate compete for the anti-LHRH antibody binding site. Since the number of sites is limited, as more sites are occupied by LHRH from the sample, fewer sites are left to bind LHRH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LHRH concentration in each sample is interpolated from this standard curve.

Small volumes of LHRH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS739715 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Luteinizing Hormone-Releasing Hormone (LHRH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LHRH. The ELISA analytical biochemical technique of the MBS739715 kit is based on LHRH antibody-LHRH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LHRH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LHRH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).