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G Protein Beta 1 (GNb1) ELISA Kit for Chicken

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS740232

Principle of the Assay: GNb1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GNb1 antibody and an GNb1-HRP conjugate. The assay sample and buffer are incubated together with GNb1-HRP conjugate in pre-coated plate for… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
G Protein Beta 1 (GNb1), ELISA Kit
Full Product Name
Chicken G Protein Beta 1 ELISA Kit
Product Gene Name
GNb1 elisa kit
Species Reactivity
Chicken
Assay Type
Competitive
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C
Molecular Weight
37,377 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (1716725..1822526, complement). Location: 1p36.33
OMIM
139380
3D Structure
ModBase 3D Structure for P62873
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Intended Uses
This GNb1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken GNb1. This ELISA kit for research use only, not for therapeutic applications!

Product Categories/Family for GNb1 elisa kit

Signal Transduction
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of GNb1 elisa kit

Chicken GNb1 ELISA Kit – Cat# MBS740232

Other Notes

Small volumes of GNb1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GNb1 purchase

MBS740232 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the G Protein Beta 1 (GNb1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GNb1. The ELISA analytical biochemical technique of the MBS740232 kit is based on GNb1 antibody-GNb1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GNb1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GNb1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GNb1. It may not necessarily be applicable to this product.

NCBI GI #
48145685
NCBI GeneID
2782
NCBI Accession #
CAG33065.1
UniProt Primary Accession #
P62873
UniProt Secondary Accession #
P04697; P04901; B1AJZ7
UniProt Related Accession #
P62873; Q1RMY8; Q5QPT1; Q71UM6; Q71UM7
NCBI Official Full Name
GNB1
NCBI Official Synonym Full Names
guanine nucleotide binding protein (G protein), beta polypeptide 1
NCBI Official Symbol
GNB1
UniProt Protein Name
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
UniProt Synonym Protein Names
Transducin beta chain 1
Protein Family
Transmembrane emp24 domain-containing protein
UniProt Gene Name
GNB1
UniProt Entry Name
GBB1_HUMAN
NCBI Protein Information
guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; transducin beta chain 1; G protein, beta-1 subunit; beta subunit, signal-transducing proteins GS/GI; guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1

NCBI Summary for GNb1

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals. [provided by RefSeq, Jul 2008]
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UniProt Comments for GNb1

G-beta 1: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the WD repeat G protein beta family.

Protein type: G protein; G protein, heterotrimeric; G protein, heterotrimeric beta

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: photoreceptor inner segment; membrane; lysosomal membrane; dendrite; plasma membrane; heterotrimeric G-protein complex; intracellular; vesicle

Molecular Function: GTPase activity; GTPase binding; protein binding; signal transducer activity; spectrin binding; protein complex binding; type 1 angiotensin receptor binding

Biological Process: phototransduction, visible light; platelet activation; acetylcholine receptor signaling, muscarinic pathway; sensory perception of taste; signal transduction; rhodopsin mediated signaling; cell proliferation; synaptic transmission; elevation of cytosolic calcium ion concentration; regulation of rhodopsin mediated signaling; retina development in camera-type eye; Ras protein signal transduction; energy reserve metabolic process; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); blood coagulation; dopamine receptor, adenylate cyclase activating pathway
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Research Articles on GNb1

1. WDR26 is a novel Gbetagamma-binding protein that is required for the efficacy of Gbetagamma signaling and leukocyte migration

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GNb1 elisa kit

 Reference Product  PubMed Publications
 GNG11 elisa kit  >1 publications with GNb1 and GNG11
 GNG2 elisa kit  >1 publications with GNb1 and GNG2
 GNAS elisa kit  >1 publications with GNb1 and GNAS

Pathways associated with GNb1 elisa kit

 Products by Pathway  Pathway Diagram
 ADP Signalling Through P2Y Purinoceptor 1 Pathway antibodies  ADP Signalling Through P2Y Purinoceptor 1 Pathway Diagram
 ADP Signalling Through P2Y Purinoceptor 12 Pathway antibodies  ADP Signalling Through P2Y Purinoceptor 12 Pathway Diagram
 Activation Of G Protein Gated Potassium Channels Pathway antibodies  Activation Of G Protein Gated Potassium Channels Pathway Diagram
 Activation Of GABAB Receptors Pathway antibodies  Activation Of GABAB Receptors Pathway Diagram
 Activation Of Kainate Receptors Upon Glutamate Binding Pathway antibodies  Activation Of Kainate Receptors Upon Glutamate Binding Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Aquaporin-mediated Transport Pathway antibodies  Aquaporin-mediated Transport Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram

Diseases associated with GNb1 elisa kit

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3 publications with GNb1 and Nervous System Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with GNb1 and Carcinoma, Hepatocellular
 Mental Disorders Antibodies  >1 publications with GNb1 and Mental Disorders
 Myocardial Infarction Antibodies  >1 publications with GNb1 and Myocardial Infarction
 Abnormalities, Drug-Induced Antibodies  >1 publications with GNb1 and Abnormalities, Drug-Induced
 Reperfusion Injury Antibodies  >1 publications with GNb1 and Reperfusion Injury
 Vision Disorders Antibodies  >1 publications with GNb1 and Vision Disorders
 Liver Diseases Antibodies  >1 publications with GNb1 and Liver Diseases
 Cardiomyopathies Antibodies  >1 publications with GNb1 and Cardiomyopathies
 Thyroid Diseases Antibodies  >1 publications with GNb1 and Thyroid Diseases

Organs/Tissues associated with GNb1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with GNb1 and Brain
 Eye Antibodies  >6 publications with GNb1 and Eye
 Muscle Antibodies  >3 publications with GNb1 and Muscle
 Ganglia Antibodies  >2 publications with GNb1 and Ganglia
 Nerve Antibodies  >1 publications with GNb1 and Nerve
 Thyroid Antibodies  >1 publications with GNb1 and Thyroid
 Ovary Antibodies  >1 publications with GNb1 and Ovary
 Heart Antibodies  >1 publications with GNb1 and Heart
 Liver Antibodies  >1 publications with GNb1 and Liver
 Embryonic Tissue Antibodies  >1 publications with GNb1 and Embryonic Tissue
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