Porcine AQP-2 ELISA Kit – Cat# MBS743419

Aquaporin 2, Collecting Duct (AQP-2) ELISA Kit for Porcine

Name: Porcine AQP-2 ELISA Kit; Aquaporin 2, Collecting Duct; MyBioSource
Brand: MyBioSource
SKU: MBS743419
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS743419

Principle of the Assay: AQP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AQP-2 antibody and an AQP-2-HRP conjugate. The assay sample and buffer are incubated together with AQP-2-HRP conjugate in pre-coated plate… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Aquaporin 2, Collecting Duct (AQP-2), ELISA Kit

Full Product Name

Porcine Aquaporin 2, Collecting Duct ELISA Kit

Product Gene Name

AQP-2 elisa kit

Species Reactivity

Porcine

Assay Type

Competitive

Samples

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Sensitivity

1.0 ng/mL.

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

28,837 Da

OMIM

gene 125800

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Intended Uses

This AQP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine AQP-2. This ELISA kit for research use only, not for therapeutic applications

Product Categories/Family for AQP-2 elisa kit

Signal Transduction

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of AQP-2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AQP-2 purchase

MBS743419 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aquaporin 2, Collecting Duct (AQP-2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AQP-2. The ELISA analytical biochemical technique of the MBS743419 kit is based on AQP-2 antibody-AQP-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AQP-2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AQP-2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AQP-2. It may not necessarily be applicable to this product.

NCBI GI #

4502179

NCBI GeneID

359

NCBI Accession #

NP_000477.1

UniProt Secondary Accession #

Q9UD68

UniProt Related Accession #

P41181

GenBank Nucleotide #

NM_000486.5

NCBI Official Full Name

aquaporin-2

NCBI Official Synonym Full Names

aquaporin 2 (collecting duct)

NCBI Official Symbol

AQP2

NCBI Official Synonym Symbols

AQP-CD; WCH-CD

UniProt Protein Name

Aquaporin-2

UniProt Synonym Protein Names

ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct

UniProt Gene Name

AQP2

UniProt Synonym Gene Names

AQP-2; AQP-CD

UniProt Entry Name

AQP2_HUMAN

NCBI Protein Information

aquaporin-2; AQP-2; aquaporin-CD; ADH water channel; water-channel aquaporin 2; collecting duct water channel protein; water channel protein for renal collecting duct

NCBI Summary for AQP-2

This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008]

UniProt Comments for AQP-2

AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter; Transporter, aquaporin family; Membrane protein, integral; Channel, misc.; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: recycling endosome; protein complex; clathrin-coated vesicle; rough endoplasmic reticulum; membrane; basolateral plasma membrane; integral to plasma membrane; lysosome; early endosome; apical plasma membrane; plasma membrane; trans-Golgi network

Molecular Function: water transporter activity; glycerol transmembrane transporter activity; actin binding; water channel activity; glycerol channel activity; PDZ domain binding

Biological Process: glycerol transport; actin filament depolymerization; response to glucagon stimulus; cellular water homeostasis; apoptosis; water transport; response to lithium ion; response to lipopolysaccharide; cellular response to water deprivation; female pregnancy; excretion; response to salt stress; response to starvation; renal water transport; hyperosmotic response; renal water homeostasis; response to calcium ion; positive regulation of calcium ion transport; transmembrane transport; aging

Disease: Diabetes Insipidus, Nephrogenic, Autosomal

Research Articles on AQP-2

1. These observations provide a framework for understanding why mutations in AQP2 cause nephrogenic diabetes insipidus.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AQP-2 elisa kit

Reference Product	PubMed Publications
AQP3 elisa kit	>219 publications with AQP-2 and AQP3
AVP elisa kit	>218 publications with AQP-2 and AVP
AQP4 elisa kit	>139 publications with AQP-2 and AQP4
SLC12A1 elisa kit	>40 publications with AQP-2 and SLC12A1
MYO5B elisa kit	>1 publications with AQP-2 and MYO5B

Pathways associated with AQP-2 elisa kit

Products by Pathway	Pathway Diagram
Aquaporin-mediated Transport Pathway antibodies	Aquaporin-mediated Transport Pathway Diagram
Passive Transport By Aquaporins Pathway antibodies	Passive Transport By Aquaporins Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram
Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway antibodies	Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway Diagram
Vasopressin-regulated Water Reabsorption Pathway antibodies	Vasopressin-regulated Water Reabsorption Pathway Diagram
Vasopressin-regulated Water Reabsorption Pathway antibodies	Vasopressin-regulated Water Reabsorption Pathway Diagram

Diseases associated with AQP-2 elisa kit

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>333 publications with AQP-2 and Kidney Diseases
Diabetes Insipidus, Nephrogenic Antibodies	>247 publications with AQP-2 and Diabetes Insipidus, Nephrogenic
Polyuria Antibodies	>135 publications with AQP-2 and Polyuria
Disease Models, Animal Antibodies	>93 publications with AQP-2 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>72 publications with AQP-2 and Cardiovascular Diseases
Heart Failure Antibodies	>55 publications with AQP-2 and Heart Failure
Hypertension Antibodies	>52 publications with AQP-2 and Hypertension
Heart Diseases Antibodies	>48 publications with AQP-2 and Heart Diseases
Brain Diseases Antibodies	>38 publications with AQP-2 and Brain Diseases
Renal Insufficiency Antibodies	>33 publications with AQP-2 and Renal Insufficiency

Organs/Tissues associated with AQP-2 elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>889 publications with AQP-2 and Kidney
Brain Antibodies	>76 publications with AQP-2 and Brain
Eye Antibodies	>26 publications with AQP-2 and Eye
Testis Antibodies	>9 publications with AQP-2 and Testis
Prostate Antibodies	>4 publications with AQP-2 and Prostate