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BAG Family Molecular Chaperone Regulator 1 (BAG1) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS744342

Intended Uses: This BAG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BAG1. This ELISA kit for research use only! More details

$585 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BAG family molecular chaperone regulator 1 (BAG1), ELISA Kit
Full Product Name
Mouse BAG family molecular chaperone regulator 1 (BAG1) ELISA Kit
Product Gene Name
BAG1 elisa kit
Species Reactivity
Mouse
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Molecular Weight
25,989 Da
OMIM
601497
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of BAG1 elisa kit

Mouse BAG1 ELISA Kit – Cat# MBS744342

Other Notes

Small volumes of BAG1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BAG1 purchase

MBS744342 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BAG family molecular chaperone regulator 1 (BAG1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BAG1. The ELISA analytical biochemical technique of the MBS744342 kit is based on BAG1 antibody-BAG1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BAG1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BAG1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for BAG1. It may not necessarily be applicable to this product.

NCBI GI #
288915525
NCBI GeneID
573
NCBI Accession #
NP_004314.5
UniProt Secondary Accession #
O75315; Q14414; Q53H32; Q5VZE8; Q5VZE9; Q5VZF0; Q96TG2; Q9Y2V4
UniProt Related Accession #
Q99933
GenBank Nucleotide #
NM_004323.5
NCBI Official Full Name
BAG family molecular chaperone regulator 1 isoform BAG-1L
NCBI Official Synonym Full Names
BCL2-associated athanogene
NCBI Official Symbol
BAG1
NCBI Official Synonym Symbols
HAP; BAG-1; RAP46
UniProt Protein Name
BAG family molecular chaperone regulator 1
UniProt Synonym Protein Names
Bcl-2-associated athanogene 1
Protein Family
BAG family molecular chaperone regulator
UniProt Gene Name
BAG1
UniProt Synonym Gene Names
HAP; BAG-1
UniProt Entry Name
BAG1_HUMAN
NCBI Protein Information
BAG family molecular chaperone regulator 1; Bcl-2-binding protein; receptor-associated protein, 46-KD; Bcl-2 associating athanogene-1 protein; glucocortoid receptor-associated protein RAP46

NCBI Summary for BAG1

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]
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UniProt Comments for BAG1

BAG1: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. Homodimer. Forms a heteromeric complex with HSP70/HSC70. Binds to the ATPase domain of HSP/HSC70 chaperones. Isoform 1, isoform 3 and isoform 4 but not isoform 2 interact with HSPA8/HSC70. Interacts with NR3C1. Interacts with the N-terminal region of STK19. Interacts with PPP1R15A. Interacts with BCL2 in an ATP-dependent manner. Isoform 2 does not interact with BCL2. Up-regulated during differentiation of bladder epithelial cells and down-regulated during differentiation of prostate epithelium. Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9p12

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; chaperone binding; receptor signaling protein activity

Biological Process: cell surface receptor linked signal transduction; apoptosis; negative regulation of apoptosis
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Research Articles on BAG1

1. BAG-1M and HspBP1 had differential impacts on the dynamic composition of steroid receptor folding complexes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with BAG1 elisa kit

 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram

Diseases associated with BAG1 elisa kit

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >16 publications with BAG1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >10 publications with BAG1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >9 publications with BAG1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >4 publications with BAG1 and Adenocarcinoma
 Cardiovascular Diseases Antibodies  >3 publications with BAG1 and Cardiovascular Diseases
 Prostatic Neoplasms Antibodies  >3 publications with BAG1 and Prostatic Neoplasms
 Nerve Degeneration Antibodies  >3 publications with BAG1 and Nerve Degeneration
 Carcinoma, Squamous Cell Antibodies  >2 publications with BAG1 and Carcinoma, Squamous Cell
 Heart Diseases Antibodies  >2 publications with BAG1 and Heart Diseases
 Neoplasms, Experimental Antibodies  >2 publications with BAG1 and Neoplasms, Experimental

Organs/Tissues associated with BAG1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >126 publications with BAG1 and Muscle
 Nerve Antibodies  >49 publications with BAG1 and Nerve
 Brain Antibodies  >42 publications with BAG1 and Brain
 Blood Antibodies  >24 publications with BAG1 and Blood
 Embryonic Tissue Antibodies  >14 publications with BAG1 and Embryonic Tissue
 Heart Antibodies  >9 publications with BAG1 and Heart
 Eye Antibodies  >8 publications with BAG1 and Eye
 Lung Antibodies  >6 publications with BAG1 and Lung
 Liver Antibodies  >5 publications with BAG1 and Liver
 Prostate Antibodies  >4 publications with BAG1 and Prostate
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