Chicken GRP78 ELISA Kit – Cat# MBS744753

Glucose Regulated Protein 78 (GRP78) ELISA Kit for Chicken

Name: Chicken GRP78 ELISA Kit; Glucose Regulated Protein 78; MyBioSource
Brand: MyBioSource
SKU: MBS744753
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS744753

Intended Uses: This GRP78 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken GRP78. This ELISA kit for research use only, not for therapeutic or test applications!Principle of the Assay: GRP78 ELISA kit applies… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Glucose regulated protein 78 (GRP78), ELISA Kit

Full Product Name

Chicken Glucose regulated protein 78 ELISA Kit

Product Gene Name

GRP78 elisa kit

Species Reactivity

Chicken

Assay Type

Quantitative Competitive

Samples

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Sensitivity

0.1 ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of GRP78. No significant cross-reactivity or interference between GRP78 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GRP78 and all the analogues, therefore, cross reaction may still exist in some cases.

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

72,333 Da

OMIM

138120

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Product Categories/Family for GRP78 elisa kit

Neurobiology

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of GRP78 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GRP78 purchase

MBS744753 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glucose regulated protein 78 (GRP78) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GRP78. The ELISA analytical biochemical technique of the MBS744753 kit is based on GRP78 antibody-GRP78 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GRP78 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GRP78. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GRP78. It may not necessarily be applicable to this product.

NCBI GI #

386758

NCBI GeneID

3309

NCBI Accession #

AAA52614.1

UniProt Secondary Accession #

Q2EF78; Q9NPF1; Q9UK02; B0QZ61

UniProt Related Accession #

P11021

NCBI Official Full Name

GRP78, partial

NCBI Official Synonym Full Names

heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

NCBI Official Symbol

HSPA5

NCBI Official Synonym Symbols

BIP; MIF2; GRP78; HEL-S-89n

UniProt Protein Name

78 kDa glucose-regulated protein

UniProt Synonym Protein Names

Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

Protein Family

78 kDa glucose-regulated protein

UniProt Gene Name

HSPA5

UniProt Synonym Gene Names

GRP78; GRP-78; BiP

UniProt Entry Name

GRP78_HUMAN

NCBI Protein Information

78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; epididymis secretory sperm binding protein Li 89n; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78

NCBI Summary for GRP78

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

UniProt Comments for GRP78

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: signalosome; endoplasmic reticulum membrane; focal adhesion; smooth endoplasmic reticulum; cell surface; mitochondrion; endoplasmic reticulum lumen; endoplasmic reticulum; ER-Golgi intermediate compartment; membrane; plasma membrane; melanosome; integral to endoplasmic reticulum membrane; midbody; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; ATPase activity; ribosome binding; misfolded protein binding; calcium ion binding; glycoprotein binding; ATP binding

Biological Process: platelet activation; ER-associated protein catabolic process; cerebellum structural organization; unfolded protein response; cerebellar Purkinje cell layer development; cellular response to glucose starvation; unfolded protein response, activation of signaling protein activity; substantia nigra development; cellular protein metabolic process; platelet degranulation; positive regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; ER overload response; positive regulation of cell migration; negative regulation of apoptosis

Research Articles on GRP78

1. The secreted recombinant human BiP possesses native amino acid sequence and structural integrity, is biologically active and without yeast-derived modifications.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GRP78 elisa kit

Reference Product	PubMed Publications
ATF6 elisa kit	>29 publications with GRP78 and ATF6
ATF4 elisa kit	>26 publications with GRP78 and ATF4
ERN1 elisa kit	>8 publications with GRP78 and ERN1
HYOU1 elisa kit	>7 publications with GRP78 and HYOU1
SIL1 elisa kit	>5 publications with GRP78 and SIL1
EIF2AK3 elisa kit	>2 publications with GRP78 and EIF2AK3

Pathways associated with GRP78 elisa kit

Products by Pathway	Pathway Diagram
ATF6-alpha Activates Chaperone Genes Pathway antibodies	ATF6-alpha Activates Chaperone Genes Pathway Diagram
ATF6-alpha Activates Chaperones Pathway antibodies	ATF6-alpha Activates Chaperones Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies	Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies	Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
IRE1alpha Activates Chaperones Pathway antibodies	IRE1alpha Activates Chaperones Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram

Diseases associated with GRP78 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>59 publications with GRP78 and Cardiovascular Diseases
Disease Models, Animal Antibodies	>56 publications with GRP78 and Disease Models, Animal
Nervous System Diseases Antibodies	>55 publications with GRP78 and Nervous System Diseases
Inflammation Antibodies	>33 publications with GRP78 and Inflammation
Liver Diseases Antibodies	>21 publications with GRP78 and Liver Diseases
Heart Diseases Antibodies	>20 publications with GRP78 and Heart Diseases
Necrosis Antibodies	>19 publications with GRP78 and Necrosis
Kidney Diseases Antibodies	>13 publications with GRP78 and Kidney Diseases
Fibrosis Antibodies	>10 publications with GRP78 and Fibrosis
Fatty Liver Antibodies	>10 publications with GRP78 and Fatty Liver

Organs/Tissues associated with GRP78 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>75 publications with GRP78 and Brain
Muscle Antibodies	>48 publications with GRP78 and Muscle
Blood Antibodies	>45 publications with GRP78 and Blood
Heart Antibodies	>38 publications with GRP78 and Heart
Liver Antibodies	>38 publications with GRP78 and Liver
Kidney Antibodies	>30 publications with GRP78 and Kidney
Nerve Antibodies	>20 publications with GRP78 and Nerve
Vascular Antibodies	>20 publications with GRP78 and Vascular
Pancreas Antibodies	>16 publications with GRP78 and Pancreas
Lung Antibodies	>14 publications with GRP78 and Lung