Cytochrome B5 (CYTB5) ELISA Kit for Human

Name: Human CYTB5 ELISA Kit; Cytochrome B5; MyBioSource
Brand: MyBioSource
SKU: MBS746232
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS746232

MBS746232 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome b5 (CYTB5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Cytochrome b5 (CYTB5), ELISA Kit

Full Product Name

Human Cytochrome b5 ELISA Kit

Product Gene Name

CYTB5 elisa kit

Species Reactivity

Human

Assay Type

Competitive

Samples

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Detection Range

1.0-25ng/mL

Sensitivity

0.1ng/mL

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

14,169 Da

OMIM

613218

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of CYTB5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CYTB5. It may not necessarily be applicable to this product.

NCBI GI #

41281768

NCBI GeneID

1528

NCBI Accession #

NP_683725.1

UniProt Secondary Accession #

Q6IB14; A8MV91; F8WEU4

UniProt Related Accession #

P00167

GenBank Nucleotide #

NM_148923.3

NCBI Official Full Name

cytochrome b5 isoform 1

NCBI Official Synonym Full Names

cytochrome b5 type A (microsomal)

NCBI Official Symbol

CYB5A

NCBI Official Synonym Symbols

CYB5; MCB5

UniProt Protein Name

Cytochrome b5

UniProt Synonym Protein Names

Microsomal cytochrome b5 type A; MCB5

Protein Family

Cytochrome b5 domain-containing protein

UniProt Gene Name

CYB5A

UniProt Synonym Gene Names

CYB5; MCB5

UniProt Entry Name

CYB5_HUMAN

NCBI Protein Information

cytochrome b5; type 1 cyt-b5

NCBI Summary for CYTB5

The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

UniProt Comments for CYTB5

CYB5A: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. Belongs to the cytochrome b5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; integral to membrane

Molecular Function: cytochrome-c oxidase activity; enzyme binding; aldo-keto reductase activity; metal ion binding; heme binding

Biological Process: vitamin metabolic process; response to cadmium ion; L-ascorbic acid metabolic process; water-soluble vitamin metabolic process

Disease: Methemoglobinemia Type Iv

Research Articles on CYTB5

1. Cytochrome b5 seems to play the key role in the formation of lipid-radical cycles in membranes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CYTB5 elisa kit

Reference Product	PubMed Publications
CYB5R3 elisa kit	>8 publications with CYTB5 and CYB5R3
CYP1A1 elisa kit	>3 publications with CYTB5 and CYP1A1
POR elisa kit	>3 publications with CYTB5 and POR
CYP3A4 elisa kit	>2 publications with CYTB5 and CYP3A4

Pathways associated with CYTB5 elisa kit

Products by Pathway	Pathway Diagram
Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies	Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
Defective BTD Causes Biotidinase Deficiency Pathway antibodies	Defective BTD Causes Biotidinase Deficiency Pathway Diagram
Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies	Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies	Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies	Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram
Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies	Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram
Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies	Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram
Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies	Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram
Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies	Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram
Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway antibodies	Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway Diagram

Diseases associated with CYTB5 elisa kit

Disease Name	Pubmed Publications
Drug-Related Side Effects and Adverse Reactions Antibodies	>2 publications with CYTB5 and Drug-Related Side Effects and Adverse Reactions
Breast Neoplasms Antibodies	>2 publications with CYTB5 and Breast Neoplasms
Hyperplasia Antibodies	>2 publications with CYTB5 and Hyperplasia
Adenocarcinoma Antibodies	>1 publications with CYTB5 and Adenocarcinoma
Weight Gain Antibodies	>1 publications with CYTB5 and Weight Gain
Carcinoma, Hepatocellular Antibodies	>1 publications with CYTB5 and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>1 publications with CYTB5 and Liver Diseases
Prenatal Exposure Delayed Effects Antibodies	>1 publications with CYTB5 and Prenatal Exposure Delayed Effects
Kidney Diseases Antibodies	>1 publications with CYTB5 and Kidney Diseases
Neoplasm Metastasis Antibodies	>1 publications with CYTB5 and Neoplasm Metastasis

Organs/Tissues associated with CYTB5 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>6 publications with CYTB5 and Liver
Testis Antibodies	>5 publications with CYTB5 and Testis
Adrenal Gland Antibodies	>3 publications with CYTB5 and Adrenal Gland
Kidney Antibodies	>3 publications with CYTB5 and Kidney
Embryonic Tissue Antibodies	>2 publications with CYTB5 and Embryonic Tissue
Blood Antibodies	>2 publications with CYTB5 and Blood
Placenta Antibodies	>1 publications with CYTB5 and Placenta
Intestine Antibodies	>1 publications with CYTB5 and Intestine
Bladder Antibodies	>1 publications with CYTB5 and Bladder
Prostate Antibodies	>1 publications with CYTB5 and Prostate