Beta2-Glycoprotein 1antibody IgG (beta2-GP1 Ab IgG) ELISA Kit for Mouse

Intended Uses: This B2GPIgG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse B2GPIgG. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: B2GPIgG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-B2GPIgG antibody and an B2GPIgG-HRP conjugate. The assay sample and buffer are incubated together with B2GPIgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the B2GPIgG concentration since B2GPIgG from samples and B2GPIgG-HRP conjugate compete for the anti-B2GPIgG antibody binding site. Since the number of sites is limited, as more sites are occupied by B2GPIgG from the sample, fewer sites are left to bind B2GPIgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The B2GPIgG concentration in each sample is interpolated from this standard curve.

Small volumes of beta2-GP1 Ab IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS748250 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the beta2-Glycoprotein 1antibody IgG (beta2-GP1 Ab IgG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing beta2-GP1 Ab IgG. The ELISA analytical biochemical technique of the MBS748250 kit is based on beta2-GP1 Ab IgG antibody-beta2-GP1 Ab IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect beta2-GP1 Ab IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, beta2-GP1 Ab IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).