Programmed Cell Death 1 Ligand 1 (PDL1/B7 H1/CD274) ELISA Kit for Human

Intended Uses: This PDL1/CD274 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PDL1/CD274. This ELISA kit for research use only!

Principle of the Assay||PDL1/CD274 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-PDL1/CD274 antibody and an PDL1/CD274-HRP conjugate. The assay sample and buffer are incubated together with PDL1/CD274-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDL1/CD274 concentration since PDL1/CD274 from samples and PDL1/CD274-HRP conjugate compete for the anti-PDL1/CD274 antibody binding site. Since the number of sites is limited, as more sites are occupied by PDL1/CD274 from the sample, fewer sites are left to bind PDL1/CD274-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDL1/CD274 concentration in each sample is interpolated from this standard curve.

Small volumes of PDL1/B7 H1/CD274 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS752680 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Programmed cell death 1 ligand 1 (PDL1/B7 H1/CD274) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDL1/B7 H1/CD274. The ELISA analytical biochemical technique of the MBS752680 kit is based on PDL1/B7 H1/CD274 antibody-PDL1/B7 H1/CD274 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDL1/B7 H1/CD274 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDL1/B7 H1/CD274. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).