Anti-Presynaptic Membrane Receptor (PsmR) ELISA Kit for Porcine

Principle of the Assay: PsmRAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Presynaptic Membrane Receptor antigen and an PsmRAb-HRP conjugate. The assay sample and buffer are incubated together with PsmRAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PsmRAb concentration since PsmRAb from samples and PsmRAb-HRP conjugate compete for the Presynaptic Membrane Receptor antigen binding site. Since the number of sites is limited, as more sites are occupied by PsmRAb from the sample, fewer sites are left to bind PsmRAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PsmRAb concentration in each sample is interpolated from this standard curve.

Small volumes of PsmR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS752841 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Presynaptic Membrane Receptor Antibody (PsmR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PsmR. The ELISA analytical biochemical technique of the MBS752841 kit is based on PsmR antibody-PsmR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PsmR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PsmR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).