25-Hydroxy Vitamin D (25OHVD) ELISA Kit for Porcine

Intended Uses: This 25-OHVD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine 25-OHVD. This ELISA kit for research use only!

Principle of the Assay||25-OHVD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-25-OHVD antibody and an 25-OHVD-HRP conjugate. The assay sample and buffer are incubated together with 25-OHVD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 25-OHVD concentration since 25-OHVD from samples and 25-OHVD-HRP conjugate compete for the anti-25-OHVD antibody binding site. Since the number of sites is limited, as more sites are occupied by 25-OHVD from the sample, fewer sites are left to bind 25-OHVD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 25-OHVD concentration in each sample is interpolated from this standard curve.

Small volumes of 25OHVD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS755169 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 25-Hydroxy vitamin D (25OHVD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 25OHVD. The ELISA analytical biochemical technique of the MBS755169 kit is based on 25OHVD antibody-25OHVD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 25OHVD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 25OHVD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).