Granzyme K (GZMK) ELISA Kit for Human

Background: Granzyme K is a serine protease produced by cytotoxic lymphocytes, including natural killer cells and cytotoxic T lymphocytes. It induces apoptosis in target cells by cleaving specific substrates, contributing to immune surveillance and defense against infected or transformed cells.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti GZMK antibody was precoated onto the 96-well plate. The biotin conjugated anti GZMK antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with GZMK conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of GZMK in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Small volumes of GZMK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS760160 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Granzyme K (GZMK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GZMK. The ELISA analytical biochemical technique of the MBS760160 kit is based on GZMK antibody-GZMK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GZMK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GZMK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).