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Glycosaminoglycan Xylosylkinase (FAM20B) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS760189

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-FAM20B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FAM20B antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Glycosaminoglycan xylosylkinase (FAM20B), ELISA Kit
Full Product Name
Human Glycosaminoglycan xylosylkinase ELISA Kit
Product Gene Name
FAM20B elisa kit
Product Synonym Names
FAM20B/Glycosaminoglycan xylosylkinase/Protein FAM20B/Xylose kinase/gxk1/XYLK
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
46,432 Da
OMIM
611063
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of FAM20B elisa kit

Human FAM20B ELISA Kit – Cat# MBS760189

Typical Standard Curve of FAM20B elisa kit

Human FAM20B ELISA Kit, Typical Standard Curve – Cat# MBS760189

Other Notes

Small volumes of FAM20B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FAM20B purchase

MBS760189 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glycosaminoglycan xylosylkinase (FAM20B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FAM20B. The ELISA analytical biochemical technique of the MBS760189 kit is based on FAM20B antibody-FAM20B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FAM20B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FAM20B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FAM20B. It may not necessarily be applicable to this product.

NCBI GI #
7662150
NCBI GeneID
9917
NCBI Accession #
NP_055679.1
UniProt Secondary Accession #
Q5W0C3; Q5W0C4
UniProt Related Accession #
O75063
GenBank Nucleotide #
NM_014864.3
NCBI Official Full Name
glycosaminoglycan xylosylkinase
NCBI Official Synonym Full Names
family with sequence similarity 20 member B
NCBI Official Symbol
FAM20B
NCBI Official Synonym Symbols
gxk1
UniProt Protein Name
Glycosaminoglycan xylosylkinase
UniProt Synonym Protein Names
Xylose kinase
Protein Family
Glycosaminoglycan xylosylkinase
UniProt Gene Name
FAM20B
UniProt Entry Name
XYLK_HUMAN
NCBI Protein Information
glycosaminoglycan xylosylkinase

UniProt Comments for FAM20B

FAM20B: Responsible for the 2-O-phosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature GAG chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG- protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1- O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-o-phosphorylation may influence the catalytic activity of B3GAT3 (GlcAT-I) which completes the precursor tetrasaccharide of GAG-protein linkage regions on which the repeating disaccharide region is synthesized. Belongs to the FAM20 family.

Protein type: EC 2.7.1.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; Golgi membrane; integral to membrane; nucleoplasm

Molecular Function: ATP binding; kinase activity; metal ion binding; phosphotransferase activity, alcohol group as acceptor

Biological Process: phosphorylation
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Research Articles on FAM20B

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FAM20B elisa kit

 Reference Product  PubMed Publications
 XYLT1 elisa kit  >1 publications with FAM20B and XYLT1

Diseases associated with FAM20B elisa kit

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >1 publications with FAM20B and Liver Diseases
 Kidney Diseases Antibodies  >1 publications with FAM20B and Kidney Diseases

Organs/Tissues associated with FAM20B elisa kit

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >4 publications with FAM20B and Bone
 Liver Antibodies  >2 publications with FAM20B and Liver
 Kidney Antibodies  >1 publications with FAM20B and Kidney
 Mouth Antibodies  >1 publications with FAM20B and Mouth
 Connective Tissue Antibodies  >1 publications with FAM20B and Connective Tissue
 Muscle Antibodies  >1 publications with FAM20B and Muscle
 Skin Antibodies  >1 publications with FAM20B and Skin
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