Melanocyte Protein PMEL (PMEL) ELISA Kit for Human

Name: Human PMEL ELISA Kit; Melanocyte Protein PMEL; MyBioSource
Brand: MyBioSource
SKU: MBS760206
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS760206

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Melanocyte protein PMEL, ELISA Kit

Full Product Name

Human Melanocyte protein PMEL ELISA Kit

Product Gene Name

PMEL elisa kit

Product Synonym Names

PMEL/P100/P1/Melanoma-associated ME20 antigen/Melanocytes lineage-specific antigen GP100/Silver locus protein homolog/Premelanosome protein/ME20-M (ME20M)/Melanocyte protein Pmel 17/PMEL17/SILV

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

0.156-10ng/ml

Sensitivity

0.094ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of PMEL. No significant cross-reactivity or interference between PMEL and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

CV<10%

Intra-assay Precision

CV<8%

Molecular Weight

66,729 Da

OMIM

155550

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of PMEL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PMEL purchase

MBS760206 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Melanocyte protein PMEL, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PMEL. The ELISA analytical biochemical technique of the MBS760206 kit is based on PMEL antibody-PMEL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PMEL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PMEL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PMEL. It may not necessarily be applicable to this product.

NCBI GI #

318037595

NCBI GeneID

6490

NCBI Accession #

NP_001186983.1

UniProt Secondary Accession #

Q12763; Q14448; Q14817; Q16565; B3KS57; B7Z6D7

UniProt Related Accession #

P40967

GenBank Nucleotide #

NM_001200054.1

NCBI Official Full Name

melanocyte protein PMEL isoform 1 preproprotein

NCBI Official Synonym Full Names

premelanosome protein

NCBI Official Symbol

PMEL

NCBI Official Synonym Symbols

P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E

UniProt Protein Name

Melanocyte protein PMEL

UniProt Synonym Protein Names

ME20-M; ME20M

Protein Family

Melanocyte protein

UniProt Gene Name

PMEL

UniProt Synonym Gene Names

D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S

UniProt Entry Name

PMEL_HUMAN

NCBI Protein Information

melanocyte protein PMEL

NCBI Summary for PMEL

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

UniProt Comments for PMEL

SILV: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. Belongs to the PMEL/NMB family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q13-q14

Cellular Component: endoplasmic reticulum membrane; extracellular region; Golgi apparatus; integral to membrane; melanosome; plasma membrane

Molecular Function: protein binding

Biological Process: melanin biosynthetic process; melanosome organization and biogenesis

Research Articles on PMEL

1. Data indicate that repeat domain (RPT) derived from Pmel17 aggregation kinetics were influenced only by lysolipid-containing phospholipid vesicles.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PMEL elisa kit

Reference Product	PubMed Publications
MLANA elisa kit	>130 publications with PMEL and MLANA
TYRP1 elisa kit	>38 publications with PMEL and TYRP1
MITF elisa kit	>28 publications with PMEL and MITF
MAGEA3 elisa kit	>25 publications with PMEL and MAGEA3
TYR elisa kit	>13 publications with PMEL and TYR

Diseases associated with PMEL elisa kit

Disease Name	Pubmed Publications
Skin Diseases Antibodies	>117 publications with PMEL and Skin Diseases
Neoplasms, Experimental Antibodies	>97 publications with PMEL and Neoplasms, Experimental
Disease Models, Animal Antibodies	>89 publications with PMEL and Disease Models, Animal
Neoplasm Metastasis Antibodies	>54 publications with PMEL and Neoplasm Metastasis
Necrosis Antibodies	>17 publications with PMEL and Necrosis
Lung Diseases Antibodies	>16 publications with PMEL and Lung Diseases
Inflammation Antibodies	>8 publications with PMEL and Inflammation
Leukopenia Antibodies	>6 publications with PMEL and Leukopenia
Pain Antibodies	>5 publications with PMEL and Pain
Breast Neoplasms Antibodies	>5 publications with PMEL and Breast Neoplasms

Organs/Tissues associated with PMEL elisa kit

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>132 publications with PMEL and Skin
Lung Antibodies	>24 publications with PMEL and Lung
Eye Antibodies	>23 publications with PMEL and Eye
Spleen Antibodies	>19 publications with PMEL and Spleen
Brain Antibodies	>16 publications with PMEL and Brain
Kidney Antibodies	>12 publications with PMEL and Kidney
Liver Antibodies	>10 publications with PMEL and Liver
Bone Antibodies	>10 publications with PMEL and Bone
Testis Antibodies	>8 publications with PMEL and Testis
Muscle Antibodies	>8 publications with PMEL and Muscle