Eosinophil Peroxidase (EPX) ELISA Kit for Human

Background: Eosinophil peroxidase is a secreted hemoprotein that catalyzes oxidations of various substrates by hydrogen peroxide. Together with other leukocyte-derived enzymes, such as Myeloperoxidase,EPX plays an important role in the pathogenesis of asthma by mediating oxidative events that lead to the production of ROS (reactive oxygen species) and RNS (reactive nitrogen species). Diseases associated with EPX include Eosinophil Peroxidase Deficiency and Proctitis.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti EPX antibody was precoated onto the 96-well plate. The biotin conjugated anti EPX antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with EPX conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of EPX in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Small volumes of EPX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS760340 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Eosinophil peroxidase (EPX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EPX. The ELISA analytical biochemical technique of the MBS760340 kit is based on EPX antibody-EPX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EPX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EPX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).