Dopamine Transporter (DAT) ELISA Kit for Human

Name: Human DAT ELISA Kit; Dopamine Transporter; MyBioSource
Brand: MyBioSource
SKU: MBS760426
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS760426

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DAT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- DAT antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Dopamine Transporter (DAT), ELISA Kit

Full Product Name

Human Dopamine Transporter ELISA Kit

Product Gene Name

DAT elisa kit

Product Synonym Names

DAT/Dopamine Transporter

Species Reactivity

Human

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

0.156-10ng/ml

Sensitivity

0.094ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of DAT. No significant cross-reactivity or interference between DAT and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DAT were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DAT were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

68,495 Da

OMIM

126455

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of DAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DAT purchase

MBS760426 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dopamine Transporter (DAT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DAT. The ELISA analytical biochemical technique of the MBS760426 kit is based on DAT antibody-DAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DAT. It may not necessarily be applicable to this product.

NCBI GI #

7108463

NCBI GeneID

6531

NCBI Accession #

AAC50179.2

UniProt Secondary Accession #

Q14996; A2RUN4

UniProt Related Accession #

Q01959

NCBI Official Full Name

dopamine transporter

NCBI Official Synonym Full Names

solute carrier family 6 (neurotransmitter transporter), member 3

NCBI Official Symbol

SLC6A3

NCBI Official Synonym Symbols

DAT; DAT1; PKDYS

UniProt Protein Name

Sodium-dependent dopamine transporter

UniProt Synonym Protein Names

Solute carrier family 6 member 3

Protein Family

D-alanine aminotransferase

UniProt Gene Name

SLC6A3

UniProt Synonym Gene Names

DAT1; DA transporter; DAT

UniProt Entry Name

SC6A3_HUMAN

NCBI Protein Information

sodium-dependent dopamine transporter

NCBI Summary for DAT

This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]

UniProt Comments for DAT

DAT: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI). It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.

Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15.3

Cellular Component: axon; cell soma; cell surface; cytoplasm; flotillin complex; integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: dopamine binding; dopamine transmembrane transporter activity; dopamine:sodium symporter activity; drug binding; monoamine transmembrane transporter activity; protease binding; protein binding; protein complex binding; protein N-terminus binding; protein phosphatase 2A binding; receptor binding

Biological Process: adenohypophysis development; aging; dopamine biosynthetic process; dopamine catabolic process; dopamine transport; dopamine uptake; lactation; locomotory behavior; monoamine transport; neurotransmitter biosynthetic process; positive regulation of multicellular organism growth; prepulse inhibition; regulation of dopamine metabolic process; response to cAMP; response to cocaine; response to drug; response to ethanol; response to iron ion; response to nicotine; sensory perception of smell; synaptic transmission; transmembrane transport

Disease: Parkinsonism-dystonia, Infantile; Tobacco Addiction, Susceptibility To

Research Articles on DAT

1. As a conclusion, there was no association between 5-HTT, DAT1 and DRD4 genes VNTR polymorphisms and obesity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DAT elisa kit

Reference Product	PubMed Publications
DRD4 elisa kit	>320 publications with DAT and DRD4
DRD2 elisa kit	>216 publications with DAT and DRD2
SLC18A2 elisa kit	>135 publications with DAT and SLC18A2
SNCA elisa kit	>27 publications with DAT and SNCA
PICK1 elisa kit	>9 publications with DAT and PICK1
CAMK2A elisa kit	>3 publications with DAT and CAMK2A
NEDD4 elisa kit	>2 publications with DAT and NEDD4

Pathways associated with DAT elisa kit

Products by Pathway	Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alpha-synuclein Signaling Pathway antibodies	Alpha-synuclein Signaling Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Cocaine Addiction Pathway antibodies	Cocaine Addiction Pathway Diagram
Cocaine Addiction Pathway antibodies	Cocaine Addiction Pathway Diagram
Dopamine Clearance From The Synaptic Cleft Pathway antibodies	Dopamine Clearance From The Synaptic Cleft Pathway Diagram
Dopaminergic Synapse Pathway antibodies	Dopaminergic Synapse Pathway Diagram
Dopaminergic Synapse Pathway antibodies	Dopaminergic Synapse Pathway Diagram

Diseases associated with DAT elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>1357 publications with DAT and Nervous System Diseases
Brain Diseases Antibodies	>1141 publications with DAT and Brain Diseases
Movement Disorders Antibodies	>984 publications with DAT and Movement Disorders
Parkinsonian Disorders Antibodies	>850 publications with DAT and Parkinsonian Disorders
Parkinson Disease Antibodies	>680 publications with DAT and Parkinson Disease
Attention Deficit Disorder with Hyperactivity Antibodies	>508 publications with DAT and Attention Deficit Disorder with Hyperactivity
Disease Models, Animal Antibodies	>358 publications with DAT and Disease Models, Animal
Schizophrenia Antibodies	>297 publications with DAT and Schizophrenia
Poisoning Antibodies	>207 publications with DAT and Poisoning
Cocaine-Related Disorders Antibodies	>207 publications with DAT and Cocaine-Related Disorders

Organs/Tissues associated with DAT elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3580 publications with DAT and Brain
Ganglia Antibodies	>238 publications with DAT and Ganglia
Kidney Antibodies	>141 publications with DAT and Kidney
Lung Antibodies	>26 publications with DAT and Lung
Testis Antibodies	>4 publications with DAT and Testis
Thymus Antibodies	>3 publications with DAT and Thymus
Uncharacterized Tissue Antibodies	>1 publications with DAT and Uncharacterized Tissue