Protein Wnt-7b (WNT7B) ELISA Kit for Human

Background: Wnt-7b is a secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid-modified and are ligands for members of the frizzled family of receptors, which mediates cell-cell communication during development. Diseases associated with WNT7B include Microphthalmia, Syndromic 9 and Macs Syndrome. Among its related pathways are ncRNAs involved in Wnt signaling in hepatocellular carcinoma and Wnt Pathway.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti WNT7B antibody was pre-coated onto the 96-well plate. The biotin conjugated anti WNT7B antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with WNT7B conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of WNT7B in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Small volumes of WNT7B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS760479 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Wnt-7b (WNT7B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT7B. The ELISA analytical biochemical technique of the MBS760479 kit is based on WNT7B antibody-WNT7B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT7B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT7B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).