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Proteinase-Activated Receptor 4 (F2RL3) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS760652

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human F2RL3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human F2RL3 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Proteinase-activated receptor 4 (F2RL3), ELISA Kit
Full Product Name
Human Proteinase-activated receptor 4 ELISA Kit
Product Gene Name
F2RL3 elisa kit
Product Synonym Names
F2RL3/Coagulation factor II receptor-like 3/Thrombin receptor-like 3/
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.781-50ng/ml
Sensitivity
0.469ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
41,133 Da
OMIM
602779
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of F2RL3 elisa kit

Human F2RL3 ELISA Kit – Cat# MBS760652

Typical Standard Curve of F2RL3 elisa kit

Human F2RL3 ELISA Kit, Typical Standard Curve – Cat# MBS760652

Other Notes

Small volumes of F2RL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for F2RL3 purchase

MBS760652 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proteinase-activated receptor 4 (F2RL3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F2RL3. The ELISA analytical biochemical technique of the MBS760652 kit is based on F2RL3 antibody-F2RL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F2RL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F2RL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for F2RL3. It may not necessarily be applicable to this product.

NCBI GI #
89353783
NCBI GeneID
9002
NCBI Accession #
NP_003941.2
UniProt Secondary Accession #
O76067; Q6DK42
UniProt Related Accession #
Q96RI0
GenBank Nucleotide #
NM_003950.2
NCBI Official Full Name
proteinase-activated receptor 4
NCBI Official Synonym Full Names
F2R like thrombin/trypsin receptor 3
NCBI Official Symbol
F2RL3
NCBI Official Synonym Symbols
PAR4
UniProt Protein Name
Proteinase-activated receptor 4
UniProt Synonym Protein Names
Coagulation factor II receptor-like 3; Thrombin receptor-like 3
Protein Family
Proteinase-activated receptor
UniProt Gene Name
F2RL3
UniProt Synonym Gene Names
PAR4; PAR-4
UniProt Entry Name
PAR4_HUMAN
NCBI Protein Information
proteinase-activated receptor 4

NCBI Summary for F2RL3

Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin. [provided by RefSeq, Jul 2008]
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UniProt Comments for F2RL3

PAR4: a G-protein coupled receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Plays a role in platelets activation. Thrombin requires PAR4 for full spreading on a fibrinogen matrix. Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets. A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: extracellular region; integral to plasma membrane; plasma membrane

Molecular Function: thrombin receptor activity

Biological Process: blood coagulation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); platelet activation; platelet dense granule organization and biogenesis; positive regulation of release of sequestered calcium ion into cytosol; response to wounding; signal transduction
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Research Articles on F2RL3

1. protease-activated receptor 4 and Trefoil factor 2 are expressed in human colorectal cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with F2RL3 elisa kit

 Reference Product  PubMed Publications
 F2RL1 elisa kit  >1 publications with F2RL3 and F2RL1
 TBXA2R elisa kit  >1 publications with F2RL3 and TBXA2R
 F2RL2 elisa kit  >1 publications with F2RL3 and F2RL2

Pathways associated with F2RL3 elisa kit

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram

Diseases associated with F2RL3 elisa kit

 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >1 publications with F2RL3 and Lung Diseases

Organs/Tissues associated with F2RL3 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >3 publications with F2RL3 and Lung
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