• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Cofilin 1, Non-Muscle (CFL1) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS760657

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Cfl1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Cfl1 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cofilin 1, Non-Muscle (Cfl1), ELISA Kit
Full Product Name
Mouse Cofilin 1, Non-Muscle ELISA Kit
Product Gene Name
Cfl1 elisa kit
Product Synonym Names
Cfl1/18 kDa phosphoprotein/p18/Cofilin/non-muscle isoform
Species Reactivity
Mouse
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
18,502 Da
OMIM
601442
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of Cfl1 elisa kit

Mouse CFL1 ELISA Kit – Cat# MBS760657

Typical Standard Curve of Cfl1 elisa kit

Mouse CFL1 ELISA Kit, Typical Standard Curve – Cat# MBS760657

Other Notes

Small volumes of Cfl1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cfl1 purchase

MBS760657 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cofilin 1, Non-Muscle (Cfl1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cfl1. The ELISA analytical biochemical technique of the MBS760657 kit is based on Cfl1 antibody-Cfl1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cfl1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Cfl1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Cfl1. It may not necessarily be applicable to this product.

NCBI GI #
5031635
NCBI GeneID
1072
NCBI Accession #
NP_005498.1
UniProt Secondary Accession #
Q53Y87; Q9UCA2; B3KUQ1
UniProt Related Accession #
P23528
GenBank Nucleotide #
NM_005507.2
NCBI Official Full Name
cofilin-1
NCBI Official Synonym Full Names
cofilin 1
NCBI Official Symbol
CFL1
NCBI Official Synonym Symbols
CFL; cofilin; HEL-S-15
UniProt Protein Name
Cofilin-1
UniProt Synonym Protein Names
18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform
Protein Family
Cofilin
UniProt Gene Name
CFL1
UniProt Synonym Gene Names
CFL; p18
UniProt Entry Name
COF1_HUMAN
NCBI Protein Information
cofilin-1

NCBI Summary for Cfl1

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
Show more

UniProt Comments for Cfl1

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cortical actin cytoskeleton; cytoplasm; extracellular space; focal adhesion; intercellular junction; membrane; nuclear matrix; nucleus; vesicle

Molecular Function: actin binding; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; actin filament depolymerization; axon guidance; blood coagulation; cytokinesis after mitosis; cytoskeleton organization and biogenesis; ephrin receptor signaling pathway; establishment of cell polarity; innate immune response; negative regulation of apoptosis; neural crest cell migration; neural fold formation; platelet activation; platelet degranulation; positive regulation of actin filament depolymerization; protein amino acid phosphorylation; regulation of cell morphogenesis; response to amino acid stimulus; response to virus; Rho protein signal transduction
Show more

Research Articles on Cfl1

1. Chronophin has a role in coordinating cell leading edge dynamics by controlling active cofilin levels

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Cfl1 elisa kit

 Reference Product  PubMed Publications
 SSH1 elisa kit  >7 publications with Cfl1 and SSH1
 PFN1 elisa kit  >4 publications with Cfl1 and PFN1
 WDR1 elisa kit  >2 publications with Cfl1 and WDR1
 ACTB elisa kit  >2 publications with Cfl1 and ACTB
 LIMK2 elisa kit  >1 publications with Cfl1 and LIMK2

Pathways associated with Cfl1 elisa kit

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPHB-mediated Forward Signaling Pathway antibodies  EPHB-mediated Forward Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram

Diseases associated with Cfl1 elisa kit

 Disease Name  Pubmed Publications
 Death Antibodies  >14 publications with Cfl1 and Death
 Nervous System Diseases Antibodies  >12 publications with Cfl1 and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >12 publications with Cfl1 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >10 publications with Cfl1 and Breast Neoplasms
 Disease Models, Animal Antibodies  >9 publications with Cfl1 and Disease Models, Animal
 Inflammation Antibodies  >7 publications with Cfl1 and Inflammation
 Neoplasm Invasiveness Antibodies  >5 publications with Cfl1 and Neoplasm Invasiveness
 Neoplasms, Experimental Antibodies  >4 publications with Cfl1 and Neoplasms, Experimental
 Memory Disorders Antibodies  >3 publications with Cfl1 and Memory Disorders
 Prenatal Exposure Delayed Effects Antibodies  >2 publications with Cfl1 and Prenatal Exposure Delayed Effects

Organs/Tissues associated with Cfl1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >23 publications with Cfl1 and Brain
 Muscle Antibodies  >15 publications with Cfl1 and Muscle
 Nerve Antibodies  >13 publications with Cfl1 and Nerve
 Blood Antibodies  >13 publications with Cfl1 and Blood
 Lung Antibodies  >9 publications with Cfl1 and Lung
 Kidney Antibodies  >6 publications with Cfl1 and Kidney
 Uterus Antibodies  >5 publications with Cfl1 and Uterus
 Embryonic Tissue Antibodies  >4 publications with Cfl1 and Embryonic Tissue
 Vascular Antibodies  >4 publications with Cfl1 and Vascular
 Lymph Node Antibodies  >4 publications with Cfl1 and Lymph Node
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000